Table 2. Used oligonucleotides and PCR... | American Society of Hematology

Table 2.

Used oligonucleotides and PCR conditions

Primers	Sequence	Amplicon size, base pairs	Annealing temperature, elongation time	Free MgCl₂
U522 (exon 3)	5′-CAA TGT GGC TGA ACA AAG GA-3′	420	60°C, 25 s	4 mM
L922 (exon 5)	5′-CCA GCA ACG ATA CCC ATG-3′
G.257 (exon 2)	5′-GGA AGA GGA GAC ACG GAA CA-3′	G1-988; G2,4-712; G3-436; G5-1110; G6-834	60°C, 90 s	2 mM
G.1225 (3′-UT)	5′-TGA GAC AGA GAC GGA GAC AT-3′
GAP1U19	5′-GAA GGT GAA GGT CGG AGT C-3′	224	55°C, 10 s	4 mM
GAP206L19	5′-GAA GAT GGT GAT GGG ATT T-3′

Primers	Sequence	Amplicon size, base pairs	Annealing temperature, elongation time	Free MgCl₂
U522 (exon 3)	5′-CAA TGT GGC TGA ACA AAG GA-3′	420	60°C, 25 s	4 mM
L922 (exon 5)	5′-CCA GCA ACG ATA CCC ATG-3′
G.257 (exon 2)	5′-GGA AGA GGA GAC ACG GAA CA-3′	G1-988; G2,4-712; G3-436; G5-1110; G6-834	60°C, 90 s	2 mM
G.1225 (3′-UT)	5′-TGA GAC AGA GAC GGA GAC AT-3′
GAP1U19	5′-GAA GGT GAA GGT CGG AGT C-3′	224	55°C, 10 s	4 mM
GAP206L19	5′-GAA GAT GGT GAT GGG ATT T-3′