Proliferation by purified adult PB CD27+, CD27−, and CB B cells
| . | [3H]thymidine incorporation (cpm) . | ||
|---|---|---|---|
| Adult PB CD27+ B cells . | Adult PB CD27− B cells . | CB B cells . | |
| Medium | 136 ± 353-152 | 136 ± 33 | 175 ± 38 |
| CD403-150/CD32T3-151 | 1513 ± 178 | 1185 ± 289 | 1759 ± 359 |
| SAC3-150 + IL-23-150 | 5370 ± 1245 | 4728 ± 956 | 8083 ± 1747 |
| SAC + IL-2 + IL-103-150 + CD40/CD32T | 15 369 ± 3013 | 11 080 ± 2574 | 14 366 ± 2984 |
| IL-43-150 | 1338 ± 348 | 989 ± 261 | 1294 ± 335 |
| IL-4 + CD40/CD32T | 4810 ± 1328 | 4170 ± 1248 | 7007 ± 1512 |
| . | [3H]thymidine incorporation (cpm) . | ||
|---|---|---|---|
| Adult PB CD27+ B cells . | Adult PB CD27− B cells . | CB B cells . | |
| Medium | 136 ± 353-152 | 136 ± 33 | 175 ± 38 |
| CD403-150/CD32T3-151 | 1513 ± 178 | 1185 ± 289 | 1759 ± 359 |
| SAC3-150 + IL-23-150 | 5370 ± 1245 | 4728 ± 956 | 8083 ± 1747 |
| SAC + IL-2 + IL-103-150 + CD40/CD32T | 15 369 ± 3013 | 11 080 ± 2574 | 14 366 ± 2984 |
| IL-43-150 | 1338 ± 348 | 989 ± 261 | 1294 ± 335 |
| IL-4 + CD40/CD32T | 4810 ± 1328 | 4170 ± 1248 | 7007 ± 1512 |
Proliferation assay, using 1 × 105 per well of highly purified adult PB CD27+, CD27−, and CB B cells, was performed as described in “Materials and methods.”
The final concentrations of SAC, IL-2, IL-10, IL-4, and anti-CD40 were 0.01%, 50 U/mL, 50 ng/mL, 50 ng/mL, and 1 μg/mL, respectively.
The transfectants accounted for 40% of the number of B cells added.
Results are expressed as the mean ± SD of triplicate experiments.