IgE production by purified adult PB CD27+, CD27−, and CB B cells
| . | Adult PB CD27+ B cells . | Adult PB CD27− B cells . | CB B cells . |
|---|---|---|---|
| Medium | < 8 | < 8 | < 8 |
| IL-4 | < 8 | < 8 | < 8 |
| IL-4* + CD40* | 26 ± 8‡ | 16 ± 4 | 18 ± 5 |
| IL-4 + CD40/CD32T† | 67 ± 14 | 49 ± 15 | 53 ± 12 |
| . | Adult PB CD27+ B cells . | Adult PB CD27− B cells . | CB B cells . |
|---|---|---|---|
| Medium | < 8 | < 8 | < 8 |
| IL-4 | < 8 | < 8 | < 8 |
| IL-4* + CD40* | 26 ± 8‡ | 16 ± 4 | 18 ± 5 |
| IL-4 + CD40/CD32T† | 67 ± 14 | 49 ± 15 | 53 ± 12 |
Immunoglobulin E concentration is given in ng/mL. IgE production assay, using 1 × 105 per well of highly purified adult PB CD27+, CD27−, and CB B cells, was performed as described in “Materials and methods.”
The final concentrations of IL-4 and anti-CD40 were 50 ng/mL and 1 μg/mL, respectively.
The transfectants accounted for 40% of the number of B cells added.
Results are expressed as the mean ± SD of triplicate experiments.