FVIIII secretion from cells in culture
| Endothelial cell type . | Culture supernate FVIII (ng/mL) . |
|---|---|
| HUVEC | 1 ± 3 |
| MVEC | 0 ± 0 |
| wtBOEC | 5 ± 4 |
| gfpBOEC | 4 ± 8 |
| BOEC transient transfection | 104 ± 1 |
| tBOEC (clone pcF8G-6) | 22 ± 5 |
| Endothelial cell type . | Culture supernate FVIII (ng/mL) . |
|---|---|
| HUVEC | 1 ± 3 |
| MVEC | 0 ± 0 |
| wtBOEC | 5 ± 4 |
| gfpBOEC | 4 ± 8 |
| BOEC transient transfection | 104 ± 1 |
| tBOEC (clone pcF8G-6) | 22 ± 5 |
FVIII secretion levels are indicated for supernate from 5 × 105 cultured BOECs after 72 hours (n = 4 each). Levels for transiently and stably transfected BOECs were significantly increased over those of background culture medium (P < .001), whereas those for the untransfected BOECs were not (though wtBOECs approached significance at P = .055).