Table 1.

Chemokine production by adenovirus-infected or LPS-treated human monocytes

TreatmentMIP-1αMIP-1βRANTES
Donor 1    
 SFM/G < .062 < .062 < .016 
 Plus adenovirus-GFP 40 79 14  
 Plus LPS 74 181 61  
Donor 2    
 SFM/G < .062 < .062 < .016 
 Plus adenovirus-GFP 15 46 12  
 Plus LPS 30 163 16 
TreatmentMIP-1αMIP-1βRANTES
Donor 1    
 SFM/G < .062 < .062 < .016 
 Plus adenovirus-GFP 40 79 14  
 Plus LPS 74 181 61  
Donor 2    
 SFM/G < .062 < .062 < .016 
 Plus adenovirus-GFP 15 46 12  
 Plus LPS 30 163 16 

Human CD14+ monocytes were cultured for 36 hours in macrophage serum-free medium and granulocyte-macrophage colony-stimulating factor and then were infected with adenovirus-GFP (200 plaque-forming units per cell) or treated with lipopolysaccharide (50 ng/mL). At 48 hours later, supernatants were assayed for the indicated chemokines. Results are expressed in nanograms per milliliter.

MIP indicates macrophage inflammatory protein; SFM/G, macrophage serum-free medium and granulocyte-macrophage colony-stimulating factor; GFP, green fluorescent protein; and LPS, lipopolysaccharide.

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