Table 2. List of differentially... | American Society of Hematology

Table 2.

List of differentially expressed genes between Rh^loLin^−/loSca-1⁺c-kit⁺and Rh^hiLin^−/loSca-1⁺c-kit⁺HSCs

Name	Clone code	Accession number	Background	Rh^lo	Rh^hi	Library
Transcription factor				Fold change	Fold change
Nfix	BA-RA6D21	NM_010906	Nuclear factor I family; role in development	3.5		B
Hoxa9	BA-RA1C45	M28449	Homologous to the Drosophila Hoxa9		2.4	B
Zfp207	BA-RA1D05	NM_011751	Zinc finger protein Zep; 2 C2H2 ZF, NLS	2.1		B
Enx-1	BA-RA1C35	NM_007971	Polycomb family, regulate Hox gene expression		2.5	B
Kinase
PI3 kinase	BA-RA5A82	BG354681	Phosphoinositide 3-kinase	2		B
PKA-like	BA-RA4C80	BG354682	cAMP-dependent protein kinase	3.1		B
Phosphatase
Tyr phosphatase	BA-RA1D25	X63440	p19 protein-tyrosine-phosphatase		2.3	B
RNA binding
RBM3	MSC-RA1A25	NM_016809	RNA binding motif 3	4.1		A
Napor-3	BA-RA6B66	AF090697	Apoptosis-related RNA-binding protein	2.7		B
Poly(a) binding	BA-RA6B35	X65553	RNA-binding protein	2.3		B
Protease
Calpain 4	MSC-RA17B72	NM_009795	Calcium-dependent protease	6.2		A
Cell growth
Activin beta C	MSC-RA15B58	X82540	TGF-beta family		4.3	A
TAX responsive	MSC-RA15B86	X81987	Malignancy-related protein 107		3.5	A
Inhibitor of CDC42	MSC-RA16A75	L07918	Hematopoietic-specific, GDP dissociation inhibitor		21	A
Protein modification
CCT eta	MSC-RA12A61	Z31399	Chaperonin containing TCP-1	4.7		A
NEDD-4	MSC-RA16D13	P46935	Containing WW domain	2.1		A
Membrane associated
pRGR1	BA-RA6C77	AF041429	4 TM; unknown function		3.9	B
TM-protein	BA-RA4C93	AF082835	Type III transmembrane protein		3.7	B
Ly-6C.2	MSC-RA11C15	M18466	u-PAR domain proteins	6.1		A
Nuclear protein
PWP1-like	BA-RA5C56	L07758	WD-40, nuclear phosphoprotein		2.3	B
Npm1	BA-RA4A70	NM_008722	Nucleophosmin 1–related protein		3	B
Karyopherin	BA-RA1C82	NM_010655	Importin		5.9	B
Calcium binding
PW29	BA-RA4C42	D49429	Calcium-binding protein		3.9	B
Cam I	BA-RA6C79	M19381	Calmodulin-dependent kinase and phosphatase		5.9	B
Chromosome modeling
HAT1	BA-RA6D08	NM_003642	Histone acetyltransferase 1	2		A/B
Histone H2A	BA-RA5A12	AF171080	Chromatin structure	3.1		A/B
Set beta isoform	MSC-RA15B10	S68987	Inhibitor of HAT and PP2a		3.7	A
HMG1-related	MSC-RA17A09	U00431	Chromatin organization	2		A/B
Miscellaneous
Ant2	BA-RA2D75	AF240003	Adenine nucleotide translocase 2		4.1	B
Prg2	MSC-RA14C84	NM_008920	Major basic protein in eosinophils of bone marrow		4.9	A
Calponin 2	MSC-RA16D77	NM_007725	Actin binding protein		6.5	A
Transport-like proteins	BA-RA1D09	AY008297	RPGR-interacting protein	2		A/B
CYLN2	BA-RA4A42	AAF99333	Cytoplasmic linker proteins	2		B
Novel
KIAA0780	BA-RA1D26	BG354685	Unknown function		2.4	B
RP42	BA-RA2C82	BG354683	Unknown function	2		B
PTD011	BA-RA5D01	BG354684	Unknown function	2.9		B
Unknown	BA-RA3D39	BG354687	Unknown function		2.1	B
Unknown	MSC-RA16B18	BG354690	Unknown function	5.1		A
Unknown	BA-RA4A52	BG354679	Unknown function		2.3	B
Unknown	BA-RA4B58	BG354678	Unknown function	2		B
Unknown	MSC-RA15C76	BG354689	Unknown function	2		A

Name	Clone code	Accession number	Background	Rh^lo	Rh^hi	Library
Transcription factor				Fold change	Fold change
Nfix	BA-RA6D21	NM_010906	Nuclear factor I family; role in development	3.5		B
Hoxa9	BA-RA1C45	M28449	Homologous to the Drosophila Hoxa9		2.4	B
Zfp207	BA-RA1D05	NM_011751	Zinc finger protein Zep; 2 C2H2 ZF, NLS	2.1		B
Enx-1	BA-RA1C35	NM_007971	Polycomb family, regulate Hox gene expression		2.5	B
Kinase
PI3 kinase	BA-RA5A82	BG354681	Phosphoinositide 3-kinase	2		B
PKA-like	BA-RA4C80	BG354682	cAMP-dependent protein kinase	3.1		B
Phosphatase
Tyr phosphatase	BA-RA1D25	X63440	p19 protein-tyrosine-phosphatase		2.3	B
RNA binding
RBM3	MSC-RA1A25	NM_016809	RNA binding motif 3	4.1		A
Napor-3	BA-RA6B66	AF090697	Apoptosis-related RNA-binding protein	2.7		B
Poly(a) binding	BA-RA6B35	X65553	RNA-binding protein	2.3		B
Protease
Calpain 4	MSC-RA17B72	NM_009795	Calcium-dependent protease	6.2		A
Cell growth
Activin beta C	MSC-RA15B58	X82540	TGF-beta family		4.3	A
TAX responsive	MSC-RA15B86	X81987	Malignancy-related protein 107		3.5	A
Inhibitor of CDC42	MSC-RA16A75	L07918	Hematopoietic-specific, GDP dissociation inhibitor		21	A
Protein modification
CCT eta	MSC-RA12A61	Z31399	Chaperonin containing TCP-1	4.7		A
NEDD-4	MSC-RA16D13	P46935	Containing WW domain	2.1		A
Membrane associated
pRGR1	BA-RA6C77	AF041429	4 TM; unknown function		3.9	B
TM-protein	BA-RA4C93	AF082835	Type III transmembrane protein		3.7	B
Ly-6C.2	MSC-RA11C15	M18466	u-PAR domain proteins	6.1		A
Nuclear protein
PWP1-like	BA-RA5C56	L07758	WD-40, nuclear phosphoprotein		2.3	B
Npm1	BA-RA4A70	NM_008722	Nucleophosmin 1–related protein		3	B
Karyopherin	BA-RA1C82	NM_010655	Importin		5.9	B
Calcium binding
PW29	BA-RA4C42	D49429	Calcium-binding protein		3.9	B
Cam I	BA-RA6C79	M19381	Calmodulin-dependent kinase and phosphatase		5.9	B
Chromosome modeling
HAT1	BA-RA6D08	NM_003642	Histone acetyltransferase 1	2		A/B
Histone H2A	BA-RA5A12	AF171080	Chromatin structure	3.1		A/B
Set beta isoform	MSC-RA15B10	S68987	Inhibitor of HAT and PP2a		3.7	A
HMG1-related	MSC-RA17A09	U00431	Chromatin organization	2		A/B
Miscellaneous
Ant2	BA-RA2D75	AF240003	Adenine nucleotide translocase 2		4.1	B
Prg2	MSC-RA14C84	NM_008920	Major basic protein in eosinophils of bone marrow		4.9	A
Calponin 2	MSC-RA16D77	NM_007725	Actin binding protein		6.5	A
Transport-like proteins	BA-RA1D09	AY008297	RPGR-interacting protein	2		A/B
CYLN2	BA-RA4A42	AAF99333	Cytoplasmic linker proteins	2		B
Novel
KIAA0780	BA-RA1D26	BG354685	Unknown function		2.4	B
RP42	BA-RA2C82	BG354683	Unknown function	2		B
PTD011	BA-RA5D01	BG354684	Unknown function	2.9		B
Unknown	BA-RA3D39	BG354687	Unknown function		2.1	B
Unknown	MSC-RA16B18	BG354690	Unknown function	5.1		A
Unknown	BA-RA4A52	BG354679	Unknown function		2.3	B
Unknown	BA-RA4B58	BG354678	Unknown function	2		B
Unknown	MSC-RA15C76	BG354689	Unknown function	2		A

Libraries A and B were made from Lin^−/loSca-1⁺ and Lin^−/loSca-1⁺Thy-1.1^loc-kit⁺cells, respectively. The changes in expression levels (fold) between Rh^lo and Rh^hi cells are indicated in the corresponding column of Rh^lo and Rh^hi. For abbreviations see Table 1.

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