Table 1. Effects of cytokines on the... | American Society of Hematology

Table 1.

Effects of cytokines on the kinetics parameters of the Gardos channel in human erythrocytes

	V_max mmol/ 10¹³ cells per min	Ca²⁺ K_0.5 (μM)	EC₅₀
Control	0.71 ± 0.14	1.21 ± 0.7	—
IL-10	2.09 ± 0.13^*	7.0 ± 1.6^*	11 ± 8 ng/mL (204 ± 148 nM)
RANTES	0.93 ± 0.06^*	2.2 ± 0.3^*	9 ± 6 ng/mL (1.2 ± 0.8 nM)
PAF	0.77 ± 0.01^*	2.1 ± 0.4^*	15 ± 7 nM

	V_max mmol/ 10¹³ cells per min	Ca²⁺ K_0.5 (μM)	EC₅₀
Control	0.71 ± 0.14	1.21 ± 0.7	—
IL-10	2.09 ± 0.13^*	7.0 ± 1.6^*	11 ± 8 ng/mL (204 ± 148 nM)
RANTES	0.93 ± 0.06^*	2.2 ± 0.3^*	9 ± 6 ng/mL (1.2 ± 0.8 nM)
PAF	0.77 ± 0.01^*	2.1 ± 0.4^*	15 ± 7 nM

Values are means ± SE of 3 experiments in duplicate determinations. The affinity constant for intracellular Ca²⁺ (K_0.5) and the maximal velocity (V_max) were calculated from the ChTX-sensitive K⁺ influx in the presence of different cytokines, as described in “Materials and methods.” RANTES, 20 ng/mL (2.6 nM); PAF, 100 nM; IL-10, 20 ng/mL (370 nM).

Paired t test

P < .05, when compared with control conditions.

View Large

This Feature Is Available To Subscribers Only