Minigene construct mutations
| Construct . | WT sequence . | Mutated sequence . |
|---|---|---|
| UI-Ns | ATTTATTTTTAAATA | ATTTATGCATAAATA |
| UI-Mf | AAATATTTTTTGGTT | AAATATCAATTGGTT |
| UI-Ec | TTCAGTTTTTTCCCG | TTCAGTGAATTCCCG |
| UI-Bs | CCGCAAGAACAACAT | CCGCACGTACGACAT |
| UI-Xm | TTTCCCCCCTTTCAT | TTTCCCGGGTTTCAT |
| R | ACAGAAAAAGAGAGA | ACAGAATTCGAGAGA |
| C | AGAGAGAAAGACTAG | AGAGAGCTCGACTAG |
| L | ATGGTGAAAACATTT | ATGGTGTCGACATTT |
| Ps | ATTTATATCAGACAT | ATTTATAACAGACAT |
| B | TCAGACATAGCAATT | TCAGAGATCTCAATT |
| AA | GGAGGTTTGTATGAA | GGAGGTAAGTATGAA |
| DI-Cl | TGTATGAACTTGAAG | TGTATCGATTTGAAG |
| DI-Hi | ACTTGAAGCTTATTT | ACTTGATAATTATTT |
| DI-Sp | TTTCAGTTGGTTGCC | TTTCACTAGTTTGCC |
| DI-Bs | GCCTGGAACCTTCTG | GCCTGTACACTTCTG |
| DI-Bc | GCATTCTTTGCTGAT | GCATTGATCACTGAT |
| DI-Bb | TTCTGTGCTGCATTT | TTCTTTCGAACATTT |
| DI-Pm | GGTTTTGCATGCAAT | GGTTTCACGTGCAAT |
| DI-Nr | CAATTGCATGAAGGG | CAATTTCGCGAAGGG |
| DI-Pv | GACTTTAGGTTTAAA | GACTTCAGCTGTAAA |
| DI-Nd2 | ATGCTTTTGCATGTT | ATGCATATGGATGTT |
| Construct . | WT sequence . | Mutated sequence . |
|---|---|---|
| UI-Ns | ATTTATTTTTAAATA | ATTTATGCATAAATA |
| UI-Mf | AAATATTTTTTGGTT | AAATATCAATTGGTT |
| UI-Ec | TTCAGTTTTTTCCCG | TTCAGTGAATTCCCG |
| UI-Bs | CCGCAAGAACAACAT | CCGCACGTACGACAT |
| UI-Xm | TTTCCCCCCTTTCAT | TTTCCCGGGTTTCAT |
| R | ACAGAAAAAGAGAGA | ACAGAATTCGAGAGA |
| C | AGAGAGAAAGACTAG | AGAGAGCTCGACTAG |
| L | ATGGTGAAAACATTT | ATGGTGTCGACATTT |
| Ps | ATTTATATCAGACAT | ATTTATAACAGACAT |
| B | TCAGACATAGCAATT | TCAGAGATCTCAATT |
| AA | GGAGGTTTGTATGAA | GGAGGTAAGTATGAA |
| DI-Cl | TGTATGAACTTGAAG | TGTATCGATTTGAAG |
| DI-Hi | ACTTGAAGCTTATTT | ACTTGATAATTATTT |
| DI-Sp | TTTCAGTTGGTTGCC | TTTCACTAGTTTGCC |
| DI-Bs | GCCTGGAACCTTCTG | GCCTGTACACTTCTG |
| DI-Bc | GCATTCTTTGCTGAT | GCATTGATCACTGAT |
| DI-Bb | TTCTGTGCTGCATTT | TTCTTTCGAACATTT |
| DI-Pm | GGTTTTGCATGCAAT | GGTTTCACGTGCAAT |
| DI-Nr | CAATTGCATGAAGGG | CAATTTCGCGAAGGG |
| DI-Pv | GACTTTAGGTTTAAA | GACTTCAGCTGTAAA |
| DI-Nd2 | ATGCTTTTGCATGTT | ATGCATATGGATGTT |
Deviations from the WT sequences are underlined.