Antibodies used to detect cell surface antigens
| Antigen . | Antibody/use* . | Source† . | Recognition . |
|---|---|---|---|
| PrP | 6H4 (fl, cyt, WBs) | 1 | |
| PrP | R29 (cryo) | 1 | |
| PrP | 3F4 (paraffin) | 2 | |
| CD3 | 3D4 (cryo) | 3 | T cells |
| CD11c | KB40 (cryo) | 3 | DC and macrophage |
| CD13 | WM47 (cryo) | 3 | Myeloid origin (DC and macrophage) |
| CD20 | L26 (cryo) | 3 | B cells |
| CD21 | CR4/23 (cryo) | 3 | FDCs (CD21 isoform) |
| CD68 | PGM1 (cryo) | 3 | Macrophages (high) |
| CD83 | HB15 (cryo) | 3 | DCs |
| HLA II | DK22 (cyt) | 2 |
| Antigen . | Antibody/use* . | Source† . | Recognition . |
|---|---|---|---|
| PrP | 6H4 (fl, cyt, WBs) | 1 | |
| PrP | R29 (cryo) | 1 | |
| PrP | 3F4 (paraffin) | 2 | |
| CD3 | 3D4 (cryo) | 3 | T cells |
| CD11c | KB40 (cryo) | 3 | DC and macrophage |
| CD13 | WM47 (cryo) | 3 | Myeloid origin (DC and macrophage) |
| CD20 | L26 (cryo) | 3 | B cells |
| CD21 | CR4/23 (cryo) | 3 | FDCs (CD21 isoform) |
| CD68 | PGM1 (cryo) | 3 | Macrophages (high) |
| CD83 | HB15 (cryo) | 3 | DCs |
| HLA II | DK22 (cyt) | 2 |
fl indicates indirect immunofluorescence and flow cytometry; cyro, indirect immunofluorescence on frozen sections; paraffin, indirect immunofluorescence on paraffin section; WBs, Western blots.
Antibodies used in the detection of PrPc in tissues or slide preparations, together with antibodies used for cellular recognition. A wider range of antibodies for the characterization of DCs by indirect immunofluorescence is given in “Materials and methods” under generation and assessment of DCs.
Sources of antibodies were 1, Prionics (Zurich, Switzerland); 2, Dako (Cambridge, United Kingdom); and 3, LRF Immunodiagnostics Unit (Oxford, United Kingdom).