Quantitative analysis of EGFP expression in human T lymphocytes transduced by lentiviral vectors
| Experiment . | LTR . | LTR-LCR . | Δ . | Δ-LCR . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| % . | MFI . | CV . | % . | MFI . | CV . | % . | MFI . | CV . | % . | MFI . | CV . | |
| 1 | 1.8 | 657 | 168 | 0.3 | 669 | 164 | 1.3 | 512 | 105 | 0.2 | 609 | 119 |
| 2 | 16.7 | 674 | 156 | 0.1 | 689 | 202 | 1.84 | 549 | 100 | 0.2 | 622 | 133 |
| 3 | 2.3 | 663 | 132 | 0.1 | 657 | 135 | 0.7 | 549 | 88 | 0.1 | 589 | 84 |
| 4 | 1.6 | 660 | 176 | 0.3 | 735 | 194 | 2.8 | 564 | 113 | 0.3 | 645 | 135 |
| 5 | 4.5 | 750 | 155 | ND | ND | ND | 1.1 | 600 | 92 | 0.5 | 654 | 87 |
| Mean | 5.38 | 6813-150 | 157 | 0.20 | 688 | 174 | 1.55 | 5553-151 | 100 | 0.26 | 6243-150,3-151 | 112 |
| SD | 6.43 | 39.2 | 16.7 | 0.12 | 34.3 | 30.6 | 0.81 | 31.7 | 10 | 0.15 | 26.4 | 24.6 |
| Experiment . | LTR . | LTR-LCR . | Δ . | Δ-LCR . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| % . | MFI . | CV . | % . | MFI . | CV . | % . | MFI . | CV . | % . | MFI . | CV . | |
| 1 | 1.8 | 657 | 168 | 0.3 | 669 | 164 | 1.3 | 512 | 105 | 0.2 | 609 | 119 |
| 2 | 16.7 | 674 | 156 | 0.1 | 689 | 202 | 1.84 | 549 | 100 | 0.2 | 622 | 133 |
| 3 | 2.3 | 663 | 132 | 0.1 | 657 | 135 | 0.7 | 549 | 88 | 0.1 | 589 | 84 |
| 4 | 1.6 | 660 | 176 | 0.3 | 735 | 194 | 2.8 | 564 | 113 | 0.3 | 645 | 135 |
| 5 | 4.5 | 750 | 155 | ND | ND | ND | 1.1 | 600 | 92 | 0.5 | 654 | 87 |
| Mean | 5.38 | 6813-150 | 157 | 0.20 | 688 | 174 | 1.55 | 5553-151 | 100 | 0.26 | 6243-150,3-151 | 112 |
| SD | 6.43 | 39.2 | 16.7 | 0.12 | 34.3 | 30.6 | 0.81 | 31.7 | 10 | 0.15 | 26.4 | 24.6 |
Lentiviral vectors were produced in 293T cells by the triple-transfection protocol. Anti-CD3-activated human T lymphocytes were infected with LTR, LTR-LCR, Δ, and Δ-LCR lentiviral vectors and analyzed by FACS 72 hours after transduction. The percentage of EGFP+ cells for each construct (%), MFI, and CV are indicated.
ND indicates not determined.
Statistically significant differences (P < .05) according to the Student t test in the MFI values between LESN-transduced and LESNΔ-LCR–transduced cells.
Statistically significant differences (P < .05) according to the Student t test in the MFI values between LESNΔ-transduced and LESNΔ-LCR–transduced cells.