Comparison of the GlcNAc-transferase activities of the enzymes expressed from the I,Ii1, andIi2 cDNAs
| . | pSecTaq2A . | pSecTaq2A-I . | pSecTaq2A-Ii1 . | pSecTaq2A-Ii2 . |
|---|---|---|---|---|
| GlcNAc transferred (pmol) | 36.0 ± 26.3 | 841.4 ± 61.0 | 38.2 ± 34.8 | 37.8 ± 21.9 |
| . | pSecTaq2A . | pSecTaq2A-I . | pSecTaq2A-Ii1 . | pSecTaq2A-Ii2 . |
|---|---|---|---|---|
| GlcNAc transferred (pmol) | 36.0 ± 26.3 | 841.4 ± 61.0 | 38.2 ± 34.8 | 37.8 ± 21.9 |
Results are the average and SD of 4 tests. Endogenous transfer of GlcNAc in the absence of acceptor substrate was corrected for each test. Amounts of the transferred GlcNAc in the mock control, pSecTaq2A, indicate the background levels of the assay, which are believed to result from the addition of the acceptor substrate.