Primer sequences used for cloning haptoglobin β and α subunit constructs
| No. . | Primer name . | Sequence . |
|---|---|---|
| 1 | F-β-1 | 5′-CGCGGATCCATCCTGGGTGGACACCTGGATGCC-3′ |
| 2 | R-β-245 | 5′-GCGGAATTCTTAGTTCTCAGCTATGGTCTTCTGAAC-3′ |
| 3 | F-β-80 | 5′-CGCGGATCCAACTACTCCCAGGTAGATATTGGGCTC-3′ |
| 4 | R-β-161 | 5′-GCGGAATTCTTACTTCTTTTCGGGGACTGTGCT-3′ |
| 5 | F-β-120 | 5′-CGCGGATTCGTTTCTGGGTGGGGGCGAAATGCC-3′ |
| 6 | R-β-140 | 5′-GCGGAATTCTTACAGCATGACATACTTCAGATG-3′ |
| 7 | F-α-1 | 5′-CGCGGATCCGTAGACTCAGGCAATGATGTCACG-3′ |
| 8 | R-α-70,129 | 5′-GCGGAATTCTTATGCTTCACATTCAGGAAGTTT-3′ |
| No. . | Primer name . | Sequence . |
|---|---|---|
| 1 | F-β-1 | 5′-CGCGGATCCATCCTGGGTGGACACCTGGATGCC-3′ |
| 2 | R-β-245 | 5′-GCGGAATTCTTAGTTCTCAGCTATGGTCTTCTGAAC-3′ |
| 3 | F-β-80 | 5′-CGCGGATCCAACTACTCCCAGGTAGATATTGGGCTC-3′ |
| 4 | R-β-161 | 5′-GCGGAATTCTTACTTCTTTTCGGGGACTGTGCT-3′ |
| 5 | F-β-120 | 5′-CGCGGATTCGTTTCTGGGTGGGGGCGAAATGCC-3′ |
| 6 | R-β-140 | 5′-GCGGAATTCTTACAGCATGACATACTTCAGATG-3′ |
| 7 | F-α-1 | 5′-CGCGGATCCGTAGACTCAGGCAATGATGTCACG-3′ |
| 8 | R-α-70,129 | 5′-GCGGAATTCTTATGCTTCACATTCAGGAAGTTT-3′ |