Table 1. Effect of chelator combinations... | American Society of Hematology

Table 1.

Effect of chelator combinations on thymocyte apoptosis

TPEN, μM	DFO or CP20, μM IBE	% apoptosis + DFO	% apoptosis + CP20
0	0	33.9 ± 4.2	33.9 ± 4.2
0.1	0	29.4 ± 1.8	25.9 ± 2.4
5	0	55.4 ± 4.2^†	46.3 ± 2.4^*
0	1	30.5 ± 3.1	33.0 ± 1.6
0	10	40.6 ± 2.2	39.4 ± 2.8
0	100	41.6 ± 3.2	55.3 ± 2.1^†
0	300	49.3 ± 5.1^*	71.1 ± 4.3^‡
0.1	1	31.4 ± 3.6	66.6 ± 5.1^‡
0.1	10	32.4 ± 2.8	76.7 ± 3.4^‡
0.1	100	34.0 ± 1.8	71.8 ± 5.4^‡
0.1	300	36.9 ± 2.2	84.9 ± 1.8^‡
5	1	45.3 ± 4.8^*	79.7 ± 2.6^‡
5	10	47.4 ± 2.8^*	83.7 ± 3.9^‡
5	100	48.0 ± 4.1^*	81.1 ± 5.3^‡
5	300	48.5 ± 3.2^*	80.7 ± 3.2^‡
50	1	51.2 ± 0.8^†	29.4 ± 4.3
50	10	54.7 ± 4.3^†	27.4 ± 2.3
50	100	48.6 ± 2.2^*	28.5 ± 2.6
50	300	52.0 ± 3.3^†	32.0 ± 1.4

TPEN, μM	DFO or CP20, μM IBE	% apoptosis + DFO	% apoptosis + CP20
0	0	33.9 ± 4.2	33.9 ± 4.2
0.1	0	29.4 ± 1.8	25.9 ± 2.4
5	0	55.4 ± 4.2^†	46.3 ± 2.4^*
0	1	30.5 ± 3.1	33.0 ± 1.6
0	10	40.6 ± 2.2	39.4 ± 2.8
0	100	41.6 ± 3.2	55.3 ± 2.1^†
0	300	49.3 ± 5.1^*	71.1 ± 4.3^‡
0.1	1	31.4 ± 3.6	66.6 ± 5.1^‡
0.1	10	32.4 ± 2.8	76.7 ± 3.4^‡
0.1	100	34.0 ± 1.8	71.8 ± 5.4^‡
0.1	300	36.9 ± 2.2	84.9 ± 1.8^‡
5	1	45.3 ± 4.8^*	79.7 ± 2.6^‡
5	10	47.4 ± 2.8^*	83.7 ± 3.9^‡
5	100	48.0 ± 4.1^*	81.1 ± 5.3^‡
5	300	48.5 ± 3.2^*	80.7 ± 3.2^‡
50	1	51.2 ± 0.8^†	29.4 ± 4.3
50	10	54.7 ± 4.3^†	27.4 ± 2.3
50	100	48.6 ± 2.2^*	28.5 ± 2.6
50	300	52.0 ± 3.3^†	32.0 ± 1.4

Thymocytes were incubated with CP20, DFO, or TPEN alone or in combination with each other for 24 hours. The cells were washed, fixed in 70% cold ethanol, and stained with propidium iodide for apoptosis measurement by flow cytometry. The data shown are the mean ± SD of 3 independent experiments done in duplicate.

P < .01, as measured by the Studentt test.

†

P < .05, as measured by the Studentt test.

‡

P < .001, as measured by the Studentt test.

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