Identification of inducible tet0-HOXA10 transgenic mice
| Mouse line . | Transgene copies . | Background expression . | Induction with tTA without doxycycline . | Leaky with tTA with doxycycline . |
|---|---|---|---|---|
| 2-5 | 60 | − | +++ | − |
| 6-2 | 10 | − | +++ | − |
| 12-5 | 5-10 | + | +++ | + |
| 13-1 | < 5 | + | ++ | + |
| 13-4 | 20 | − | + | − |
| 14-1 | 10 | + | ++ | + |
| 15-1 | 20 | − | +++ | + |
| 17-3 | 60 | − | +++ | − |
| 17-5 | < 5 | ++ | ++ | ++ |
| Mouse line . | Transgene copies . | Background expression . | Induction with tTA without doxycycline . | Leaky with tTA with doxycycline . |
|---|---|---|---|---|
| 2-5 | 60 | − | +++ | − |
| 6-2 | 10 | − | +++ | − |
| 12-5 | 5-10 | + | +++ | + |
| 13-1 | < 5 | + | ++ | + |
| 13-4 | 20 | − | + | − |
| 14-1 | 10 | + | ++ | + |
| 15-1 | 20 | − | +++ | + |
| 17-3 | 60 | − | +++ | − |
| 17-5 | < 5 | ++ | ++ | ++ |
Copy number in the tetO-HOXA10 transgenic mouse lines was estimated by Southern blot analysis. To induce transgene expression in hematopoietic cells, BM cells were transduced with the NIT-GFP vector and grown in liquid culture for 5 days with (uninduced) or without (induced) doxycycline. RNA from transduced cells, with and without induction, was analyzed by RT-PCR. The number of + signs indicates the level of HOXA10 transgene expression; + weak expression, ++ intermediate expression, and +++ strong expression.