Thymic and splenic denritic cells produced by myeloid-committed precursor populations
| Weeks posttransfer of precursors . | Total DCs per thymus (× 10−3, per 104 precursors transferred) . | Thymic DCs (× 10−3) . | Total DCs per spleen (× 10−3, per 104 precursors transferred) . | Splenic DCs (× 10−3) . | |||
|---|---|---|---|---|---|---|---|
| CD8α+ . | CD8α− . | CD4+8α− . | CD4−8α− . | CD4−8α+ . | |||
| BM GMPs | |||||||
| 1 | < 0.1 | < 0.1 | < 0.1 | 1.4 | 0.7 | 0.4 | 0.3 |
| 2 | 1.0 | 0.8 | 0.2 | 8.1 | 2.2 | 0.8 | 5.1 |
| 3 | 0.5 | 0.3 | 0.2 | 6.1 | 2.7 | 1.5 | 1.9 |
| 4 | 0.4 | 0.3 | 0.1 | 4.3 | 2.0 | 1.5 | 0.8 |
| BM CMPs | |||||||
| 1 | 1.2 | 0.8 | 0.4 | 169.6 | 48.6 | 43.5 | 77.5 |
| 2 | 8.8 | 6.2 | 2.6 | 270.1 | 59.4 | 23.7 | 187.0 |
| 3 | 4.0 | 2.6 | 1.4 | 354.8 | 170.1 | 52.2 | 132.5 |
| 4 | 1.5 | 1.2 | 0.3 | 154.0 | 68.0 | 22.0 | 64.0 |
| Weeks posttransfer of precursors . | Total DCs per thymus (× 10−3, per 104 precursors transferred) . | Thymic DCs (× 10−3) . | Total DCs per spleen (× 10−3, per 104 precursors transferred) . | Splenic DCs (× 10−3) . | |||
|---|---|---|---|---|---|---|---|
| CD8α+ . | CD8α− . | CD4+8α− . | CD4−8α− . | CD4−8α+ . | |||
| BM GMPs | |||||||
| 1 | < 0.1 | < 0.1 | < 0.1 | 1.4 | 0.7 | 0.4 | 0.3 |
| 2 | 1.0 | 0.8 | 0.2 | 8.1 | 2.2 | 0.8 | 5.1 |
| 3 | 0.5 | 0.3 | 0.2 | 6.1 | 2.7 | 1.5 | 1.9 |
| 4 | 0.4 | 0.3 | 0.1 | 4.3 | 2.0 | 1.5 | 0.8 |
| BM CMPs | |||||||
| 1 | 1.2 | 0.8 | 0.4 | 169.6 | 48.6 | 43.5 | 77.5 |
| 2 | 8.8 | 6.2 | 2.6 | 270.1 | 59.4 | 23.7 | 187.0 |
| 3 | 4.0 | 2.6 | 1.4 | 354.8 | 170.1 | 52.2 | 132.5 |
| 4 | 1.5 | 1.2 | 0.3 | 154.0 | 68.0 | 22.0 | 64.0 |
GMPs indicate granulocyte and macrophage precursors; CMPs, common myeloid precursors. See Table 1 footnote for other abbreviations.
Purified BM CMPs or GMPs (1 to 2 × 104) were injected intravenously into lethally irradiated Ly 5.1 recipients. At various times after precursor cell transfer, donor-derived DC populations were analyzed, and the number of total donor-derived DCs and the number of each donor-derived DC population were calculated as described in Table1 and expressed per 104 cells transferred. The data presented are means of 2 to 3 such experiments at each time point. Each experimental group included 3 mice.