Thymic and splenic dendritic cells produced by lymphoid-restricted precursor populations
| Weeks posttransfer of precursors . | Total DCs per thymus (× 10−3, per 104 precursors transferred) . | Thymic DCs (× 10−3) . | Total DCs per spleen (× 10−3, per 104 precursors transferred) . | Splenic DCs (× 10−3) . | |||
|---|---|---|---|---|---|---|---|
| CD8α+ . | CD8α− . | CD4+8α− . | CD4−8α− . | CD4−8α+ . | |||
| Thymic CD4low | |||||||
| 1 | 2.8 | 1.7 | 1.1 | 60.3 | 15.0 | 7.5 | 38.0 |
| 2 | 3.6 | 2.7 | 0.9 | 92.5 | 31.0 | 6.5 | 55.0 |
| 3 | 0.4 | 0.3 | 0.1 | 15.0 | 8.1 | 3.5 | 3.4 |
| BM CLPs | |||||||
| 1 | 0.7 | 0.3 | 0.4 | 6.9 | 1.4 | 1.3 | 4.2 |
| 2 | 15.0 | 11.3 | 3.7 | 747.0 | 152.0 | 50.0 | 545.0 |
| 3 | 5.1 | 3.0 | 2.1 | 107.0 | 55.0 | 21.0 | 31.0 |
| 4 | 2.5 | 1.0 | 1.5 | 18.9 | 5.0 | 3.9 | 10.0 |
| 5 | 2.6 | 1.5 | 1.1 | 6.7 | 4.5 | 0.6 | 1.4 |
| Weeks posttransfer of precursors . | Total DCs per thymus (× 10−3, per 104 precursors transferred) . | Thymic DCs (× 10−3) . | Total DCs per spleen (× 10−3, per 104 precursors transferred) . | Splenic DCs (× 10−3) . | |||
|---|---|---|---|---|---|---|---|
| CD8α+ . | CD8α− . | CD4+8α− . | CD4−8α− . | CD4−8α+ . | |||
| Thymic CD4low | |||||||
| 1 | 2.8 | 1.7 | 1.1 | 60.3 | 15.0 | 7.5 | 38.0 |
| 2 | 3.6 | 2.7 | 0.9 | 92.5 | 31.0 | 6.5 | 55.0 |
| 3 | 0.4 | 0.3 | 0.1 | 15.0 | 8.1 | 3.5 | 3.4 |
| BM CLPs | |||||||
| 1 | 0.7 | 0.3 | 0.4 | 6.9 | 1.4 | 1.3 | 4.2 |
| 2 | 15.0 | 11.3 | 3.7 | 747.0 | 152.0 | 50.0 | 545.0 |
| 3 | 5.1 | 3.0 | 2.1 | 107.0 | 55.0 | 21.0 | 31.0 |
| 4 | 2.5 | 1.0 | 1.5 | 18.9 | 5.0 | 3.9 | 10.0 |
| 5 | 2.6 | 1.5 | 1.1 | 6.7 | 4.5 | 0.6 | 1.4 |
CLPs indicate common lymphoid precursors. See Table 1 footnote for other abbreviations.
Purified BM CLPs or the thymic CD4low precursors (1 to 2 × 104) were injected intravenously into lethally irradiated Ly 5.1 recipients. At various times after precursor cell transfer, donor-derived DC populations were analyzed, and the number of total donor-derived DCs and the number of each donor-derived DC population were calculated as described in Table 1 and expressed per 104 cells transferred. The data presented are means of 2 to 3 such experiments at each time point. Each experimental group included 3 mice.