Thymic and splenic dendritic cell reconstitution by total unseparated bone marrow cells
| Weeks posttransfer . | Total DCs per thymus (× 10−3, per 106 BM transferred) . | Thymic DCs (× 10−3) . | Total DCs per spleen (× 10−3, per 106 BM transferred) . | Splenic DCs (× 10−3) . | |||
|---|---|---|---|---|---|---|---|
| CD8α+ . | CD8α− . | CD4+8α− . | CD4−8α− . | CD4−8α+ . | |||
| 1 | 0.7 | 0.1 | 0.6 | 43 | 6 | 14 | 23 |
| 2 | 40.3 | 25.4 | 14.9 | 1556 | 293 | 330 | 933 |
| 4 | 25.9 | 11.4 | 14.5 | 703 | 426 | 174 | 103 |
| 8 | 64.0 | 38.1 | 25.9 | 636 | 357 | 170 | 108 |
| Weeks posttransfer . | Total DCs per thymus (× 10−3, per 106 BM transferred) . | Thymic DCs (× 10−3) . | Total DCs per spleen (× 10−3, per 106 BM transferred) . | Splenic DCs (× 10−3) . | |||
|---|---|---|---|---|---|---|---|
| CD8α+ . | CD8α− . | CD4+8α− . | CD4−8α− . | CD4−8α+ . | |||
| 1 | 0.7 | 0.1 | 0.6 | 43 | 6 | 14 | 23 |
| 2 | 40.3 | 25.4 | 14.9 | 1556 | 293 | 330 | 933 |
| 4 | 25.9 | 11.4 | 14.5 | 703 | 426 | 174 | 103 |
| 8 | 64.0 | 38.1 | 25.9 | 636 | 357 | 170 | 108 |
DCs indicate dendritic cells; BM, bone marrow.
Total BM cells (3 to 5 × 106) from C57BL/6 Ly 5.2 mice were intravenously injected into lethally irradiated Ly 5.1 recipient mice. At 1 to 8 weeks after BM cell transfer, DCs were enriched from the recipient thymus and spleen and then stained with fluorescent-conjugated antibodies to Ly 5.2, and DC markers CD11c, CD4, CD8α, and DEC-205. The phenotype of BM-derived DCs was determined by examining the expression of CD4 and CD8α on the donor type Ly 5.2+CD11c+ cells. The absolute number of total BM-derived DCs, as well the number of each BM-derived DC population per thymus and per spleen, was calculated at each time point and expressed per 106 BM cells transferred. The data are the means of 2 to 3 such experiments at each time point. Each experimental group contained 3 mice.