Charge analysis of N-linked oligosaccharides from human serum EPO and rhEPO
| Glycoprotein . | Neutral glycans, % . | Mono-acidic glycans, % . | Di-acidic glycans, % . | Tri-acidic glycans, % . | Tetra-acidic glycans, % . |
|---|---|---|---|---|---|
| Human serum EPO | 20 | 9 | 48 | 23 | 0 |
| EPO alfa | 4 | 5 | 41 | 31 | 19 |
| EPO beta | 3 | 2 | 17 | 32 | 46 |
| EPO omega | 3 | 10 | 34 | 32 | 21 |
| Glycoprotein . | Neutral glycans, % . | Mono-acidic glycans, % . | Di-acidic glycans, % . | Tri-acidic glycans, % . | Tetra-acidic glycans, % . |
|---|---|---|---|---|---|
| Human serum EPO | 20 | 9 | 48 | 23 | 0 |
| EPO alfa | 4 | 5 | 41 | 31 | 19 |
| EPO beta | 3 | 2 | 17 | 32 | 46 |
| EPO omega | 3 | 10 | 34 | 32 | 21 |