Expression of Ras or Bcr/Abl permits in ECoM cell lines does not prevent differentiation
| Sample . | Treatment . | Progenitors (%) . | Granulocytes (%) . | Monocytes (%) . |
|---|---|---|---|---|
| ECoM-G | Parental + E2 | 99 | 1 | 0 |
| Parental − E2 | 2 | 98 | 0 | |
| RAS + E2 | 98 | 0 | 2 | |
| RAS − E2 | 1 | 0 | 99 | |
| BCR/ABL + E2 | 98 | 0 | 2 | |
| BCR/ABL − E2 | 18 | 17 | 65 | |
| ECoM-M | Parental + E2 | 100 | 0 | 0 |
| Parental − E2 | 1 | 1 | 98 | |
| RAS + E2 | 89 | 0 | 11 | |
| RAS − E2 | 5 | 0 | 95 | |
| BCR/ABL + E2 | 97 | 0 | 3 | |
| BCR/ABL − E2 | 3 | 1 | 96 |
| Sample . | Treatment . | Progenitors (%) . | Granulocytes (%) . | Monocytes (%) . |
|---|---|---|---|---|
| ECoM-G | Parental + E2 | 99 | 1 | 0 |
| Parental − E2 | 2 | 98 | 0 | |
| RAS + E2 | 98 | 0 | 2 | |
| RAS − E2 | 1 | 0 | 99 | |
| BCR/ABL + E2 | 98 | 0 | 2 | |
| BCR/ABL − E2 | 18 | 17 | 65 | |
| ECoM-M | Parental + E2 | 100 | 0 | 0 |
| Parental − E2 | 1 | 1 | 98 | |
| RAS + E2 | 89 | 0 | 11 | |
| RAS − E2 | 5 | 0 | 95 | |
| BCR/ABL + E2 | 97 | 0 | 3 | |
| BCR/ABL − E2 | 3 | 1 | 96 |
Derivatives of ECoM-G and ECoM-M cells were established by retroviral infection with constructs encoding H-RasL61 or Bcr/Ablp190 followed by culture in the absence of GM-CSF. Granulocytic or monocytic differentiation, 7 days following the removal of estrogen, was assessed on the basis of morphology from Wright-Giemsa–stained cytocentrifuge preparations (minimum of 200 cells/sample).