Effects of mobilization by granulocyte colony-stimulating factor on the prognitors in the muscle and peripheral blood
| Colony . | PB (per mL) . | Muscle (per 2 hind legs) . | ||
|---|---|---|---|---|
| G-CSF(−) . | G-CSF(+) . | G-CSF(−) . | G-CSF(+) . | |
| BFU-E | 18 ± 6 | 216 ± 1266-150 | 3 ± 2 | 2 ± 2 |
| GEMM | 4 ± 0 | 83 ± 336-151 | 1 ± 1 | 1 ± 1 |
| GM | 42 ± 8 | 1013 ± 2566-151 | 136 ± 9 | 138 ± 11 |
| Total | 74 ± 5 | 1594 ± 2366-151 | 158 ± 15 | 157 ± 11 |
| Colony . | PB (per mL) . | Muscle (per 2 hind legs) . | ||
|---|---|---|---|---|
| G-CSF(−) . | G-CSF(+) . | G-CSF(−) . | G-CSF(+) . | |
| BFU-E | 18 ± 6 | 216 ± 1266-150 | 3 ± 2 | 2 ± 2 |
| GEMM | 4 ± 0 | 83 ± 336-151 | 1 ± 1 | 1 ± 1 |
| GM | 42 ± 8 | 1013 ± 2566-151 | 136 ± 9 | 138 ± 11 |
| Total | 74 ± 5 | 1594 ± 2366-151 | 158 ± 15 | 157 ± 11 |
Peripheral blood and muscles were collected from 6 mice that received G-CSF treatment and 6 control mice without G-CSF treatment. After Percoll density-gradient separation, mononuclear cells were cultured in methylcellulose in the presence of thrombopoietin, steel factor, erythropoietin, IL-3, granulocyte/macrophage CSF. On day 8 of culture, colonies were counted. Data represent the mean and SD of values based on the results obtained from 4 dishes. Two additional experiments showed similar results.
PB indicates peripheral blood; G-CSF, granulocyte colony-stimulating factor; BFU-E, erythroid burst-forming units; GEMM, mixed colonies containing erythroid and myeloid cells and megakaryocytes; and GM, granulocyte and/or macrophage colonies.
P < .05, as compared with the control mice.
P < .01, as compared with the control mice.