Hematopoietic reconstitution by Ly-5.1 bone marrow and muscle cells of chimeric mice
| Experiment no.4-150 . | Cells transplanted . | Radioprotective Ly-5.2 BM cells . | No. secondary Ly-5.2 recipients . | % Ly-5.1 cells in secondary Ly-5.2 recipients' PB . | Multilineage reconstitution by Ly-5.1 cells, %4-151 . | ||
|---|---|---|---|---|---|---|---|
| G/M . | T . | B . | |||||
| 5 | Muscle 1.5 × 105 | Lin−c-kit+Sca-1+CD34+‡ | 2 | 7 | 26 | 53 | 6 |
| 2 × 103 | 3 | 10 | 75 | 16 | |||
| BM 1.0 × 106 | Lin−c-kit+Sca-1+CD34+‡ | 2 | 87 | 12 | 31 | 40 | |
| 2 × 103 | 81 | ND | ND | ND | |||
| 6 | Muscle 4.0 × 105 | Unfractionated 2 × 105 | 1 | 4 | 29 | 27 | 45 |
| BM 1.0 × 106 | Unfractionated 2 × 105 | 1 | 47 | 7 | 43 | 50 | |
| Experiment no.4-150 . | Cells transplanted . | Radioprotective Ly-5.2 BM cells . | No. secondary Ly-5.2 recipients . | % Ly-5.1 cells in secondary Ly-5.2 recipients' PB . | Multilineage reconstitution by Ly-5.1 cells, %4-151 . | ||
|---|---|---|---|---|---|---|---|
| G/M . | T . | B . | |||||
| 5 | Muscle 1.5 × 105 | Lin−c-kit+Sca-1+CD34+‡ | 2 | 7 | 26 | 53 | 6 |
| 2 × 103 | 3 | 10 | 75 | 16 | |||
| BM 1.0 × 106 | Lin−c-kit+Sca-1+CD34+‡ | 2 | 87 | 12 | 31 | 40 | |
| 2 × 103 | 81 | ND | ND | ND | |||
| 6 | Muscle 4.0 × 105 | Unfractionated 2 × 105 | 1 | 4 | 29 | 27 | 45 |
| BM 1.0 × 106 | Unfractionated 2 × 105 | 1 | 47 | 7 | 43 | 50 | |
Designated numbers of muscle-derived nucleated cells or lineage-negative (Lin−) BM cells17 of chimeric mice were transplanted into lethally irradiated secondary Ly-5.2 recipient mice along with radioprotective cells. At 6 months later, PB was collected from the secondary recipients, and the levels of engraftment were analyzed by flow cytometry. Nucleated PB cells were stained with anti-Ly-5.1 and either anti-Gr-1 and anti-Mac-1, anti-Thy-1.2, or anti-B220.
BM indicates bone marrow; PB, peripheral blood; G/M, granulocytes and/or monocytes; T, T lymphocytes; B, B lymphocytes; ND, not determined.
The experimental mice correspond to experiments 5 and 6 in Table 3.
The percentages of cells within each lineage that expressed Ly-5.1.
The cells were prepared as described previously.17