Time course comparison of colony formation from different sources of hematopoietic progenitors
| Colony . | Muscle, 1.0 × 104 per dish day of colony formation . | PB, 1.2 × 105 per dish day of colony formation . | BM, 1.2 × 103 per dish day of colony formation . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 8 . | 10 . | 12 . | 14 . | 8 . | 10 . | 12 . | 14 . | 8 . | 10 . | 12 . | 14 . | |
| E | 1 | 1 | 1 | 1 | 19 | 13 | 19 | 11 | 7 | 6 | 6 | 7 |
| n | 14 | 10 | 3 | 1 | 2 | 1 | 1 | 0 | 19 | 8 | 1 | 0 |
| m | 15 | 10 | 26 | 22 | 0 | 0 | 2 | 0 | 4 | 11 | 18 | 29 |
| n/m | 20 | 11 | 9 | 6 | 4 | 0 | 2 | 5 | 15 | 17 | 15 | 13 |
| E/M | 0 | 3 | 5 | 1 | 4 | 4 | 1 | 2 | 2 | 4 | 1 | 1 |
| mast | 4 | 5 | 4 | 3 | 26 | 27 | 11 | 13 | 4 | 3 | 5 | 3 |
| mast/Bl | 0 | 0 | 0 | 1 | 21 | 34 | 22 | 51 | 1 | 0 | 0 | 0 |
| Bl | 1 | 5 | 7 | 16 | 0 | 1 | 0 | 0 | 5 | 0 | 0 | 1 |
| Others | 5 | 3 | 2 | 6 | 9 | 10 | 7 | 6 | 8 | 7 | 12 | 8 |
| Total | 60 | 48 | 57 | 57 | 85 | 90 | 65 | 88 | 65 | 56 | 58 | 62 |
| Colony . | Muscle, 1.0 × 104 per dish day of colony formation . | PB, 1.2 × 105 per dish day of colony formation . | BM, 1.2 × 103 per dish day of colony formation . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 8 . | 10 . | 12 . | 14 . | 8 . | 10 . | 12 . | 14 . | 8 . | 10 . | 12 . | 14 . | |
| E | 1 | 1 | 1 | 1 | 19 | 13 | 19 | 11 | 7 | 6 | 6 | 7 |
| n | 14 | 10 | 3 | 1 | 2 | 1 | 1 | 0 | 19 | 8 | 1 | 0 |
| m | 15 | 10 | 26 | 22 | 0 | 0 | 2 | 0 | 4 | 11 | 18 | 29 |
| n/m | 20 | 11 | 9 | 6 | 4 | 0 | 2 | 5 | 15 | 17 | 15 | 13 |
| E/M | 0 | 3 | 5 | 1 | 4 | 4 | 1 | 2 | 2 | 4 | 1 | 1 |
| mast | 4 | 5 | 4 | 3 | 26 | 27 | 11 | 13 | 4 | 3 | 5 | 3 |
| mast/Bl | 0 | 0 | 0 | 1 | 21 | 34 | 22 | 51 | 1 | 0 | 0 | 0 |
| Bl | 1 | 5 | 7 | 16 | 0 | 1 | 0 | 0 | 5 | 0 | 0 | 1 |
| Others | 5 | 3 | 2 | 6 | 9 | 10 | 7 | 6 | 8 | 7 | 12 | 8 |
| Total | 60 | 48 | 57 | 57 | 85 | 90 | 65 | 88 | 65 | 56 | 58 | 62 |
Mac-1+ cells were removed from the mononuclear cells by immunomagnetic separation17 to reduce the background monocyte/macrophage population. Designated numbers of cells were plated in quadruplicate in the presence of thrombopoietin, steel factor, erythropoietin, IL-11, and IL-3. All colonies consisting of 50 or more cells were harvested and stained with May-Grünwald-Giemsa for lineage determination.
PB indicates peripheral blood; BM, bone marrow; E, erythrocyte; n, neutrophil; m, monocyte-macrophage; M, megakaryocyte; mast, mast cell-basophil; Bl, blast cell.