Comparison of colony formation from different sources of hematopoietic progenitors
| Day-8 colonies . | Day-2 CFU-E . | |||||
|---|---|---|---|---|---|---|
| Sample . | BFU-E . | GM . | GEMM . | Mast . | Total . | |
| Muscle, (2 × 104/dish) | 1 ± 1 | 27 ± 3 | 2 ± 1 | 2 ± 1 | 34 ± 1 | 3 ± 1 |
| BM, (2 × 103/dish) | 3 ± 0 | 23 ± 2 | 2 ± 1 | 2 ± 1 | 30 ± 3 | 189 ± 12 |
| PB, (2 × 105/dish) | 8 ± 1 | 3 ± 1 | 2 ± 1 | 17 ± 1 | 32 ± 3 | 0 |
| Day-8 colonies . | Day-2 CFU-E . | |||||
|---|---|---|---|---|---|---|
| Sample . | BFU-E . | GM . | GEMM . | Mast . | Total . | |
| Muscle, (2 × 104/dish) | 1 ± 1 | 27 ± 3 | 2 ± 1 | 2 ± 1 | 34 ± 1 | 3 ± 1 |
| BM, (2 × 103/dish) | 3 ± 0 | 23 ± 2 | 2 ± 1 | 2 ± 1 | 30 ± 3 | 189 ± 12 |
| PB, (2 × 105/dish) | 8 ± 1 | 3 ± 1 | 2 ± 1 | 17 ± 1 | 32 ± 3 | 0 |
Mac-1+ cells were removed from the mononuclear cells by immunomagnetic separation17 to reduce the background monocyte/macrophage population. For day-8 colony formation, designated numbers of cells were plated in quadruplicate in the presence of thrombopoietin, steel factor, erythropoietin, IL-11, and IL-3. For CFU-E assay, 5 × 104 cells were plated per dish. Data represent the mean and SD of values obtained from 4 dishes. Two additional experiments showed similar results.
BFU-E indicates erythroid burst-forming units; GM, granulocyte and/or macrophage colonies; GEMM, mixed colonies containing erythroid and myeloid cells and megakaryocytes; CFU-E, erythroid colony-forming units; BM, bone marrow; PB, peripheral blood.