N-terminal amino acid sequence of polypeptide bands separated by SDS-PAGE of purified von Willebrand factor–cleaving protease
| Mr . | Amino acid sequence . |
|---|---|
| 350 kd unreduced | Ser-Val-Ser-Gly-Lys-Pro-Gln-Tyr-Met-Val* |
| 150 kd unreduced | Ala-Ala-Gly-Gly-Ile |
| 140 kd unreduced | Ala-Ala-Gly-Gly-Ile |
| 130 kd unreduced | Ala-Ala-Gly-Gly-Ile |
| 110 kd unreduced | Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu |
| 70 kd unreduced | Asp/Ser-Gln/Leu-Thr/Met-Val/Pro-Ser/Phe† |
| 180 kd reduced | Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu |
| 170 kd reduced | Ala-Ala-Gly-Gly-Ile |
| 160 kd reduced | Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu-Leu-Leu-Val-Ala-Val-Gly |
| 120 kd reduced | Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu-Leu-Leu-Val-Ala-Val-Gly |
| 40 kd reduced | Asp-Gln-Thr-Val-Ser† |
| Mr . | Amino acid sequence . |
|---|---|
| 350 kd unreduced | Ser-Val-Ser-Gly-Lys-Pro-Gln-Tyr-Met-Val* |
| 150 kd unreduced | Ala-Ala-Gly-Gly-Ile |
| 140 kd unreduced | Ala-Ala-Gly-Gly-Ile |
| 130 kd unreduced | Ala-Ala-Gly-Gly-Ile |
| 110 kd unreduced | Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu |
| 70 kd unreduced | Asp/Ser-Gln/Leu-Thr/Met-Val/Pro-Ser/Phe† |
| 180 kd reduced | Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu |
| 170 kd reduced | Ala-Ala-Gly-Gly-Ile |
| 160 kd reduced | Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu-Leu-Leu-Val-Ala-Val-Gly |
| 120 kd reduced | Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu-Leu-Leu-Val-Ala-Val-Gly |
| 40 kd reduced | Asp-Gln-Thr-Val-Ser† |