Purification of von Willebrand factor–cleaving protease
| . | Protein absorbance units (280 nm) . | vWF-cp activity plasma units* . | ||
|---|---|---|---|---|
| Total . | Percent of initial . | Total . | Percent of initial . | |
| Initial material: 800 mL normal plasma | 48 000 | 100 | 800 | 100 |
| First step | ||||
| IgG-AffiGel | ||||
| BioGel P-6DG | ||||
| Protein G–Sepharose | 105 | 0.22 | 400 | 50 |
| Second step | ||||
| Ig-TheraSorb | ||||
| High Q Support | ||||
| Lentil lectin–Sepharose | 3 | 0.006 | 38 | 4.8 |
| Third step | ||||
| Heparin-Sepharose | ||||
| High Q Support | ||||
| Sephacryl S-300 HR | 1.1 | 0.002 | 18 | 2.3 |
| . | Protein absorbance units (280 nm) . | vWF-cp activity plasma units* . | ||
|---|---|---|---|---|
| Total . | Percent of initial . | Total . | Percent of initial . | |
| Initial material: 800 mL normal plasma | 48 000 | 100 | 800 | 100 |
| First step | ||||
| IgG-AffiGel | ||||
| BioGel P-6DG | ||||
| Protein G–Sepharose | 105 | 0.22 | 400 | 50 |
| Second step | ||||
| Ig-TheraSorb | ||||
| High Q Support | ||||
| Lentil lectin–Sepharose | 3 | 0.006 | 38 | 4.8 |
| Third step | ||||
| Heparin-Sepharose | ||||
| High Q Support | ||||
| Sephacryl S-300 HR | 1.1 | 0.002 | 18 | 2.3 |
One plasma unit is the activity of vWF-cp in 1 mL normal human plasma.