Oligonucleotide primers used to screen for novelABO gene mutations found in this study
| Primer designation . | Reaction ID . | Primer sequence (5′ → 3′) . | Primer location in ABO gene . |
|---|---|---|---|
| JK-F3 | W1 | CATGCTGCCATAGGATCATTGC | 3-150 |
| JK-R3 | W1 | GAGCCAGGAGGTGGGTTTGC | 3-150 |
| ABO-in3F | W1 | gctggccgttacagggtctg | Intron 3 |
| ABO-203CR | W1 | AGGGAGGCACTGACATTATACG | Exon 4 |
| mo-57a | W1 | gggtttgttcctatctctttgc | Intron 5 |
| ABO-350CR | W1 | CTTGATGGCAAACACAGTTAACG | Exon 6 |
| mo-101s | W2-3 | ccgtccgcctgccttgcag | Intron 6 |
| ABO-407R-Aw | W2 | CCATGAAGTGCTTCTCCGCCA | Exon 7 |
| ABO-965R-Aw | W2 | GCTGCTGGTCCCACAAGTACC | Exon 7 |
| ABO-996R-Aw | W2 | TCCTCAGGACGGCGGGTC | Exon 7 |
| ABO-539R-Bw | W3 | CATGGACACGTCCTGCCAGT | Exon 7 |
| ABO-548R-Bw | W3 | ATGCGGCGCATGGACACGC | Exon 7 |
| ABO-721R-ABw | W3 | CTCGTAGGTGAAGGCCTCCCA | Exon 7 |
| ABO-863R-Bw | W3 | GTTGGCCTGGTCGACCATCC | Exon 7 |
| ABO-873R-Bw | W3 | CTCGATGCCGTTGGCCTGC | Exon 7 |
| ABO-1036R-Bw | W3 | CGGACCGCCTGGTGGTTCC | Exon 7 |
| ABO-1055R-Bw | W3 | GCAGCCGCTCACGGGTTCT | Exon 7 |
| Primer designation . | Reaction ID . | Primer sequence (5′ → 3′) . | Primer location in ABO gene . |
|---|---|---|---|
| JK-F3 | W1 | CATGCTGCCATAGGATCATTGC | 3-150 |
| JK-R3 | W1 | GAGCCAGGAGGTGGGTTTGC | 3-150 |
| ABO-in3F | W1 | gctggccgttacagggtctg | Intron 3 |
| ABO-203CR | W1 | AGGGAGGCACTGACATTATACG | Exon 4 |
| mo-57a | W1 | gggtttgttcctatctctttgc | Intron 5 |
| ABO-350CR | W1 | CTTGATGGCAAACACAGTTAACG | Exon 6 |
| mo-101s | W2-3 | ccgtccgcctgccttgcag | Intron 6 |
| ABO-407R-Aw | W2 | CCATGAAGTGCTTCTCCGCCA | Exon 7 |
| ABO-965R-Aw | W2 | GCTGCTGGTCCCACAAGTACC | Exon 7 |
| ABO-996R-Aw | W2 | TCCTCAGGACGGCGGGTC | Exon 7 |
| ABO-539R-Bw | W3 | CATGGACACGTCCTGCCAGT | Exon 7 |
| ABO-548R-Bw | W3 | ATGCGGCGCATGGACACGC | Exon 7 |
| ABO-721R-ABw | W3 | CTCGTAGGTGAAGGCCTCCCA | Exon 7 |
| ABO-863R-Bw | W3 | GTTGGCCTGGTCGACCATCC | Exon 7 |
| ABO-873R-Bw | W3 | CTCGATGCCGTTGGCCTGC | Exon 7 |
| ABO-1036R-Bw | W3 | CGGACCGCCTGGTGGTTCC | Exon 7 |
| ABO-1055R-Bw | W3 | GCAGCCGCTCACGGGTTCT | Exon 7 |
Polymorphic nucleotides in primer sequences are shown in bold, primer sequences targeting intron sequences in lower case. In addition to the primers in this table, mo-21F and mo-31R (Table 2) were used as the internal control primer pair in primer mixes W2 and W3.
These primer sequences correspond to exons 8 and 9 sequences in the JK blood group gene.38