IFN-γ production by bcr-abl-specific CD4+T-lymphocyte clones in response to CML-derived monocytes, immature DCs, and mature DCs
| Clone . | Donor, patient no. . | APC . | IFN-γ production, pg/mL* . |
|---|---|---|---|
| None | b3a2 CML, no. 1 | Monocytes | < 10 |
| Immature DCs | < 10 | ||
| Mature DCs | < 10 | ||
| b3a2 CML, no. 2 | Monocytes | < 10 | |
| Immature DCs | < 10 | ||
| Mature DCs | < 10 | ||
| MY-1 | b3a2 CML, no. 1 | Monocytes | < 10 |
| Immature DCs | 183 ± 25 | ||
| Mature DCs | 638 ± 87 | ||
| b3a2 CML, no. 2 | Monocytes | 35 ± 14 | |
| Immature DCs | 256 ± 41 | ||
| Mature DCs | 706 ± 36 | ||
| TO-1 | b3a2 CML, no. 1 | Monocytes | 43 ± 4 |
| Immature DCs | 251 ± 20 | ||
| Mature DCs | 821 ± 121 | ||
| b3a2 CML, no. 2 | Monocytes | < 10 | |
| Immature DCs | 153 ± 16 | ||
| Mature DCs | 688 ± 88 |
| Clone . | Donor, patient no. . | APC . | IFN-γ production, pg/mL* . |
|---|---|---|---|
| None | b3a2 CML, no. 1 | Monocytes | < 10 |
| Immature DCs | < 10 | ||
| Mature DCs | < 10 | ||
| b3a2 CML, no. 2 | Monocytes | < 10 | |
| Immature DCs | < 10 | ||
| Mature DCs | < 10 | ||
| MY-1 | b3a2 CML, no. 1 | Monocytes | < 10 |
| Immature DCs | 183 ± 25 | ||
| Mature DCs | 638 ± 87 | ||
| b3a2 CML, no. 2 | Monocytes | 35 ± 14 | |
| Immature DCs | 256 ± 41 | ||
| Mature DCs | 706 ± 36 | ||
| TO-1 | b3a2 CML, no. 1 | Monocytes | 43 ± 4 |
| Immature DCs | 251 ± 20 | ||
| Mature DCs | 821 ± 121 | ||
| b3a2 CML, no. 2 | Monocytes | < 10 | |
| Immature DCs | 153 ± 16 | ||
| Mature DCs | 688 ± 88 |
IFN-γ production by MY-1 and TO-1 in the presence of monocytes, immature DCs, and mature DCs derived from HLA-DRB1*0901+patients with b3a2 type CML was determined by ELISA. IFN-γ production by monocytes, immature DCs, and mature DCs alone was also determined. The results shown represent the means ± SDs of triplicate experiments.
The values of IFN-γ production by MY-1 and TO-1 cocultured with monocytes, immature DCs, and mature DCs generated from patients with CML were significantly different from each other (P < .01).