Concentration-dependent influence of deoxyguanosine-5′-phosphorothioate on HL-60 cell growth
| Concentration (μM) . | Cell counts ± SD . | |||
|---|---|---|---|---|
| 24 h . | 48 h . | 72 h . | 96 h . | |
| 2.5 | 99.0 ± 9.0 | 92.2 ± 5.2 | 99.1 ± 5.9 | 93.1 ± 4.9 |
| 5.0 | 108.0 ± 15.7 | 116.1 ± 10.1 | 104.7 ± 11.6 | 114.5 ± 8.5 |
| 10.0 | 107.0 ± 18.0 | 120.2 ± 13.1 | 115.3 ± 7.3 | 120.8 ± 17.7 |
| 25.0 | 112.1 ± 11.0 | 136.0 ± 9.0 | 131.4 ± 8.7 | 112.7 ± 9.5 |
| 50.0 | 131.7 ± 15.7 | 148.6 ± 8.8 | 135.0 ± 11.2 | 123.0 ± 12.2 |
| 100.0 | 153.0 ± 18.4 | 151.5 ± 18.8 | 139.0 ± 10.5 | 118.8 ± 21.2 |
| Concentration (μM) . | Cell counts ± SD . | |||
|---|---|---|---|---|
| 24 h . | 48 h . | 72 h . | 96 h . | |
| 2.5 | 99.0 ± 9.0 | 92.2 ± 5.2 | 99.1 ± 5.9 | 93.1 ± 4.9 |
| 5.0 | 108.0 ± 15.7 | 116.1 ± 10.1 | 104.7 ± 11.6 | 114.5 ± 8.5 |
| 10.0 | 107.0 ± 18.0 | 120.2 ± 13.1 | 115.3 ± 7.3 | 120.8 ± 17.7 |
| 25.0 | 112.1 ± 11.0 | 136.0 ± 9.0 | 131.4 ± 8.7 | 112.7 ± 9.5 |
| 50.0 | 131.7 ± 15.7 | 148.6 ± 8.8 | 135.0 ± 11.2 | 123.0 ± 12.2 |
| 100.0 | 153.0 ± 18.4 | 151.5 ± 18.8 | 139.0 ± 10.5 | 118.8 ± 21.2 |
Cell viability was determined with tetrazolium salt assay (expressed as a percentage of control cell counts) 72 hours after addition of deoxyguanosine-5′-phosphorothioate at a concentration-dependent manner. Cells were incubated in RPMI 1640 medium supplemented with 20% FBS. Data points represent means ± SDs of at least 3 experiments.