The influence of mononucleotides, nucleosides and purine bases on human umbilical vein endothelial cell, HeLa, K 562, and HL-60 cell growth
| . | Cell counts after 72-hour incubation ± SD . | |||
|---|---|---|---|---|
| HUVECs . | HeLa . | K 562 . | HL-60 . | |
| dAMP | 90.3 ± 6.3 | 88.0 ± 9.6 | 97.2 ± 6.9 | 98.0 ± 6.9 |
| TMP | 87.3 ± 12.7 | 71.8 ± 10.8 | 89.2 ± 6.6 | 100.3 ± 8.5 |
| dGMP | 72.8 ± 8.2 | 85.6 ± 6.3 | 97.6 ± 11.3 | 100.0 ± 12.7 |
| dCMP | 99.0 ± 1.0 | 95.0 ± 11.0 | 94.3 ± 8.7 | 98.0 ± 14.5 |
| dAMPS | 124.8 ± 5.7 | 89.2 ± 14.8 | 96.8 ± 5.2 | 120.0 ± 11.2 |
| TMPS | 153.0 ± 11.4 | 98.3 ± 12.8 | 99.5 ± 5.8 | 139.0 ± 10.2 |
| dGMPS | 133.2 ± 12.1 | 98.7 ± 11.2 | 106.3 ± 8.5 | 139.0 ± 10.5 |
| dCMPS | 117.5 ± 10.5 | 94.5 ± 6.5 | 99.7 ± 2.5 | 118.0 ± 10.8 |
| dAdenosine | 90.0 ± 6.0 | 85.5 ± 6.5 | 86.3 ± 11.7 | 103.0 ± 2.2 |
| Thymidine | 92.7 ± 1.7 | 69.0 ± 2.2 | 69.8 ± 9.4 | 103.6 ± 6.5 |
| dGuanosine | 63.5 ± 10.8 | 72.7 ± 11.2 | 11.4 ± 2.3 | 45.5 ± 9.4 |
| dCytidine | 98.5 ± 3.5 | 85.5 ± 1.5 | 94.2 ± 4.0 | 91.5 ± 2.0 |
| Guanine | 68.0 ± 10.0 | 98.0 ± 2.1 | 13.0 ± 5.1 | 39.5 ± 6.5 |
| Xanthine | 96.0 ± 5.2 | 91.0 ± 2.2 | 107.7 ± 5.2 | 92.0 ± 7.1 |
| . | Cell counts after 72-hour incubation ± SD . | |||
|---|---|---|---|---|
| HUVECs . | HeLa . | K 562 . | HL-60 . | |
| dAMP | 90.3 ± 6.3 | 88.0 ± 9.6 | 97.2 ± 6.9 | 98.0 ± 6.9 |
| TMP | 87.3 ± 12.7 | 71.8 ± 10.8 | 89.2 ± 6.6 | 100.3 ± 8.5 |
| dGMP | 72.8 ± 8.2 | 85.6 ± 6.3 | 97.6 ± 11.3 | 100.0 ± 12.7 |
| dCMP | 99.0 ± 1.0 | 95.0 ± 11.0 | 94.3 ± 8.7 | 98.0 ± 14.5 |
| dAMPS | 124.8 ± 5.7 | 89.2 ± 14.8 | 96.8 ± 5.2 | 120.0 ± 11.2 |
| TMPS | 153.0 ± 11.4 | 98.3 ± 12.8 | 99.5 ± 5.8 | 139.0 ± 10.2 |
| dGMPS | 133.2 ± 12.1 | 98.7 ± 11.2 | 106.3 ± 8.5 | 139.0 ± 10.5 |
| dCMPS | 117.5 ± 10.5 | 94.5 ± 6.5 | 99.7 ± 2.5 | 118.0 ± 10.8 |
| dAdenosine | 90.0 ± 6.0 | 85.5 ± 6.5 | 86.3 ± 11.7 | 103.0 ± 2.2 |
| Thymidine | 92.7 ± 1.7 | 69.0 ± 2.2 | 69.8 ± 9.4 | 103.6 ± 6.5 |
| dGuanosine | 63.5 ± 10.8 | 72.7 ± 11.2 | 11.4 ± 2.3 | 45.5 ± 9.4 |
| dCytidine | 98.5 ± 3.5 | 85.5 ± 1.5 | 94.2 ± 4.0 | 91.5 ± 2.0 |
| Guanine | 68.0 ± 10.0 | 98.0 ± 2.1 | 13.0 ± 5.1 | 39.5 ± 6.5 |
| Xanthine | 96.0 ± 5.2 | 91.0 ± 2.2 | 107.7 ± 5.2 | 92.0 ± 7.1 |
HUVEC indicates human umbilical endothelial cell; DAMP, deoxyadenosine-5′-phosphate; TMP, thymidine-5′-phosphate; dCMP, deoxycytidine-5′-phosphate; dAMPS, deoxyadenosine-5′-phosphorothioate; TMPS, thymidine-5′-phosphorothioate; dGMPS, deoxyguanosine-5′-phosphorothioate; dCMPS, deoxycytidine-5′-phosphorothioate; dGMP, 2′-deoxyguanosine 5′-monophosphate; and an initial d, deoxy.
Cell viability was determined with tetrazolium salt assay (expressed as a percentage of control cell counts) 72 hours after addition of mononucleotides, nucleosides, or purine bases at a concentration of 100 μM. Cells were incubated in RPMI 1640 medium supplemented with 10% (K 562, HeLa) or 20% FBS (HUVECs, HL-60). Data points represent means of at least 6 experiments ± SD.