Evaluation of intra-run variability of BCL-6gene real-time reverse transcription–polymerase chain reaction at different inputs of Raji cell RNA
| Raji cells RNA input (ng) . | Ct ± SD . | CV (%) . |
|---|---|---|
| 50 | 22.23 ± 0.05 | 0.20 |
| 20 | 23.48 ± 0.15 | 0.65 |
| 10 | 24.20 ± 0.22 | 0.88 |
| 2 | 27.50 ± 0.47 | 1.70 |
| 0.4 | 28.79 ± 0.68 | 2.36 |
| 0.08 | 31.97 ± 0.13 | 0.41 |
| Raji cells RNA input (ng) . | Ct ± SD . | CV (%) . |
|---|---|---|
| 50 | 22.23 ± 0.05 | 0.20 |
| 20 | 23.48 ± 0.15 | 0.65 |
| 10 | 24.20 ± 0.22 | 0.88 |
| 2 | 27.50 ± 0.47 | 1.70 |
| 0.4 | 28.79 ± 0.68 | 2.36 |
| 0.08 | 31.97 ± 0.13 | 0.41 |
Ct indicates threshold cycle; CV, coefficient of variation.