Leukemic engraftment detected by fluorescence in situ hybridization from transplantation of CD34low/− side population versus non–side population cells into nonobese diabetic–evere combined immunodeficient mice
| Case . | Clonal marker . | Cell fraction . | Cell no. injected (× 10−3) . | % Engrafted human cells . | No. positive nuclei/total . | % Positive nuclei . |
|---|---|---|---|---|---|---|
| 17 | Monosomy 7 | SP | 10 | 32 | 18/200 | 9 |
| non-SP | 110 | 65 | 171/200 | 85 | ||
| 9 | Monosomy 7 | SP | 100 | 45 | 180/200 | 90 |
| non-SP | — | — | — | — | ||
| 6 | Monosomy 7 | SP | 0.45 | < 0.1 | 21/27 | 77 |
| non-SP | 160 | < 0.1 | 11/13 | 84 | ||
| 18 | Monosomy 7 | SP | 0.064 | < 0.1 | 5/5 | 100 |
| non-SP | 390 | < 0.1 | 5/5 | 100 | ||
| 19 | Monosomy 7 | SP | 1.40 | < 0.1 | 6/11 | 54 |
| non-SP | 545 | 0 | — | — | ||
| 20 | Trisomy 8 | SP | 100 | 90 | 1/200 | 0.5 |
| Monosomy 15 | SP | 100 | 90 | 191/200 | 95.5 | |
| non-SP | — | — | — | — | ||
| 21 | Trisomy 8 | SP | 0.33 | < 0.1 | 0/2 | 0 |
| non-SP | — | — | — | — | ||
| 22 | Trisomy 8 | SP | 0.90 | 0 | — | — |
| non-SP | 2500 | < 0.1 | 1/4 | 25 |
| Case . | Clonal marker . | Cell fraction . | Cell no. injected (× 10−3) . | % Engrafted human cells . | No. positive nuclei/total . | % Positive nuclei . |
|---|---|---|---|---|---|---|
| 17 | Monosomy 7 | SP | 10 | 32 | 18/200 | 9 |
| non-SP | 110 | 65 | 171/200 | 85 | ||
| 9 | Monosomy 7 | SP | 100 | 45 | 180/200 | 90 |
| non-SP | — | — | — | — | ||
| 6 | Monosomy 7 | SP | 0.45 | < 0.1 | 21/27 | 77 |
| non-SP | 160 | < 0.1 | 11/13 | 84 | ||
| 18 | Monosomy 7 | SP | 0.064 | < 0.1 | 5/5 | 100 |
| non-SP | 390 | < 0.1 | 5/5 | 100 | ||
| 19 | Monosomy 7 | SP | 1.40 | < 0.1 | 6/11 | 54 |
| non-SP | 545 | 0 | — | — | ||
| 20 | Trisomy 8 | SP | 100 | 90 | 1/200 | 0.5 |
| Monosomy 15 | SP | 100 | 90 | 191/200 | 95.5 | |
| non-SP | — | — | — | — | ||
| 21 | Trisomy 8 | SP | 0.33 | < 0.1 | 0/2 | 0 |
| non-SP | — | — | — | — | ||
| 22 | Trisomy 8 | SP | 0.90 | 0 | — | — |
| non-SP | 2500 | < 0.1 | 1/4 | 25 |
2 × 102 bone marrow MNC from NOD/SCID mice transplanted with sorted CD34low/− side population (SP) or non–SP cells were analyzed by fluorescence in situ hybridization with probes (D7Z1, D8Z2, D15Z3) for the respective clonal leukemia marker. The values are numbers (percentages) of all human cells detected by hybridization. Only the results from samples with positive fluorescence in situ hybridization signals are shown. Engraftment levels were estimated by staining 2 × 105 bone marrow mononuclear cells per slide, with the use of parallel samples, for human CD45+ cells.
SP indicates side population; NA, not available.