Polymerase chain reaction sense primers for germ line gene leader regions and amplicon lengths when used with 5′ constant region antisense primers
| Germ line donor (subtype) . | Primer sequence (5′-3′) . | Amplicon length (bp) . |
|---|---|---|
| 1c (VλI) | ATG GCC AGC TTC CCT CTC CTC | ∼ 447 |
| 2a2 (VλII) | ATG GCC TGG GCT CTG CTG CTC | " |
| 3r (VλIII) | ATG GCA TGG ATC CCT CTC TTC | " |
| 6a (VλVI) | ATG GCC TGG GCT CCA CTA CTT | ∼ 435 |
| O18-O8 (VKI) | ATG GAC ATG AGG GTC CCT GCT | ∼ 414 |
| LFVK431 (VKI) | ATG GAC ATG AGA GTC CTC GCT | " |
| Germ line donor (subtype) . | Primer sequence (5′-3′) . | Amplicon length (bp) . |
|---|---|---|
| 1c (VλI) | ATG GCC AGC TTC CCT CTC CTC | ∼ 447 |
| 2a2 (VλII) | ATG GCC TGG GCT CTG CTG CTC | " |
| 3r (VλIII) | ATG GCA TGG ATC CCT CTC TTC | " |
| 6a (VλVI) | ATG GCC TGG GCT CCA CTA CTT | ∼ 435 |
| O18-O8 (VKI) | ATG GAC ATG AGG GTC CCT GCT | ∼ 414 |
| LFVK431 (VKI) | ATG GAC ATG AGA GTC CTC GCT | " |