Yield of tetramer-positive cells
| CTL no. . | CD8+ cells at onset . | Presorting . | Postsorting . | Method of sorting . | After rapid expansion . |
|---|---|---|---|---|---|
| 1 | 9 | 100 (49) | 7 (95) | Flow cytometry | 59 (81) |
| 2 | 13.8 | 95 (50) | 12.7 (94) | Flow cytometry | Failed |
| 3 | 13.8 | 95 (33) | 14.8 (99) | Paramagnetic beads | 106.9 (86) |
| 4 | 12 | 220 (28) | 22 (94.5) | Flow cytometry | 95 (75.4) |
| 5 | 10 | 150 (81) | 26.8 (99.8) | Paramagnetic beads | ND |
| CTL no. . | CD8+ cells at onset . | Presorting . | Postsorting . | Method of sorting . | After rapid expansion . |
|---|---|---|---|---|---|
| 1 | 9 | 100 (49) | 7 (95) | Flow cytometry | 59 (81) |
| 2 | 13.8 | 95 (50) | 12.7 (94) | Flow cytometry | Failed |
| 3 | 13.8 | 95 (33) | 14.8 (99) | Paramagnetic beads | 106.9 (86) |
| 4 | 12 | 220 (28) | 22 (94.5) | Flow cytometry | 95 (75.4) |
| 5 | 10 | 150 (81) | 26.8 (99.8) | Paramagnetic beads | ND |
Yield of tetramer-positive cells of 5 pp65495-503-specific CTLs that were purified by flow sorting or with anti-PE–coated paramagnetic beads. The number in the second column represents the number of CD8+ cells with which the culture was commenced. The numbers in the other columns indicate the total number of cells in culture (× 106); the percentage tetramer-positive cells is indicated in parentheses. One bead-purified pp65495-503-specific CTL lines (no. 5) was not expanded; the rapid expansion of line 2 failed probably due to technical problems during flow sorting, which increased the sorting time substantially and resulted in dead cells in the sorted population.
ND indicates not done.