Effect of monoclonal antibodies on shear-induced platelet adhesion to human collagen type I studied in a parallel-plate flow chamber
| Anti-GPIb mAb . | Surface coverage (% of control) . |
|---|---|
| 6B4 | 4.0 ± 1.2 |
| 24G10 | 6.4 ± 2.1 |
| 12G1 | 10.4 ± 4.3 |
| 12E4 | 8.1 ± 5.1 |
| 27A10 | 10.4 ± 3.1 |
| 26D1 | 90 ± 3.5 |
| Anti-GPIb mAb . | Surface coverage (% of control) . |
|---|---|
| 6B4 | 4.0 ± 1.2 |
| 24G10 | 6.4 ± 2.1 |
| 12G1 | 10.4 ± 4.3 |
| 12E4 | 8.1 ± 5.1 |
| 27A10 | 10.4 ± 3.1 |
| 26D1 | 90 ± 3.5 |
At a concentration of 5 μg/mL, all mAbs inhibited shear-induced adhesion, with the exception of the control mAb 26D1. Results are expressed as percentage maximal platelet adhesion (surface coverage) obtained in the absence of mAb (control 32.5% ± 5.2%, set as 100% surface coverage). Mean ± SE, n = 3.
GPIb indicates glycoprotein Ib; mAb, monoclonal antibody.