Tissue H and L ferritin content in adult mice
| Organ . | Age . | Fth+/+ . | Fth+/− . | ||||
|---|---|---|---|---|---|---|---|
| L subunit . | H subunit . | L/H . | L subunit . | H subunit . | L/H . | ||
| Liver | 25 weeks | 47 ± 18 | 27 ± 8 | 1.7 | 54 ± 22 | 6 ± 2 | 9 |
| 45 weeks | 120 ± 26 | 74 ± 50 | 1.6 | 160 ± 38 | 23 ± 4 | 7 | |
| Spleen | 25 weeks | 116 ± 100 | 58 ± 7 | 2 | 226 ± 81 | 16 ± 9 | 14 |
| 45 weeks | 431 ± 82 | 97 ± 56 | 4.4 | 767 ± 420 | 41 ± 6 | 18 | |
| Heart | 25 weeks | 6 ± 1.5 | 43 ± 5 | 0.14 | 8 ± 2 | 16 ± 5 | 0.5 |
| 45 weeks | 13 ± 6 | 77 ± 40 | 0.16 | 24 ± 6 | 39 ± 3 | 0.6 | |
| Organ . | Age . | Fth+/+ . | Fth+/− . | ||||
|---|---|---|---|---|---|---|---|
| L subunit . | H subunit . | L/H . | L subunit . | H subunit . | L/H . | ||
| Liver | 25 weeks | 47 ± 18 | 27 ± 8 | 1.7 | 54 ± 22 | 6 ± 2 | 9 |
| 45 weeks | 120 ± 26 | 74 ± 50 | 1.6 | 160 ± 38 | 23 ± 4 | 7 | |
| Spleen | 25 weeks | 116 ± 100 | 58 ± 7 | 2 | 226 ± 81 | 16 ± 9 | 14 |
| 45 weeks | 431 ± 82 | 97 ± 56 | 4.4 | 767 ± 420 | 41 ± 6 | 18 | |
| Heart | 25 weeks | 6 ± 1.5 | 43 ± 5 | 0.14 | 8 ± 2 | 16 ± 5 | 0.5 |
| 45 weeks | 13 ± 6 | 77 ± 40 | 0.16 | 24 ± 6 | 39 ± 3 | 0.6 | |
H and L ferritin content were determined by enzyme-linked immunosorbent assay, with the use of subunit-specific antibodies, and recombinant mouse H or L ferritin for calibration. Results are the mean ± SD of values obtained for three 25-week-old or three 45-week-old animals of each genotype and are expressed as μg recombinant ferritin per gram wet tissues.