Table 5. Host CD4 cells are required for... | American Society of Hematology

Table 5.

Host CD4⁺ cells are required for engraftment promotion by anti-CD154 monoclonal antibody

mAb	No. chimeric (day 44)	% donor (day 44)	No. chimeric (day 119)	% donor (day 119)
None	0/10	0	0/10	0
Anti-CD154	10/10	57 ± 7^5-150	10/10	68 ± 6^5-150
Depleting anti-CD4	0/9	0^5-151	ND	ND
Depleting anti-CD8	0/10	0^5-151	ND	ND
Depleting anti-CD4 + anti-CD8	10/10	31 ± 25^5-150,^5-151	0/10	1 ± 1^5-150,^5-151
Anti-CD154 + depleting anti-CD4	2/10	8 ± 19^5-151	2/10	11 ± 23^5-151
Anti-CD154 + depleting anti-CD8	10/10	61 ± 7^5-150	10/10	72 ± 9^5-150

mAb	No. chimeric (day 44)	% donor (day 44)	No. chimeric (day 119)	% donor (day 119)
None	0/10	0	0/10	0
Anti-CD154	10/10	57 ± 7^5-150	10/10	68 ± 6^5-150
Depleting anti-CD4	0/9	0^5-151	ND	ND
Depleting anti-CD8	0/10	0^5-151	ND	ND
Depleting anti-CD4 + anti-CD8	10/10	31 ± 25^5-150,^5-151	0/10	1 ± 1^5-150,^5-151
Anti-CD154 + depleting anti-CD4	2/10	8 ± 19^5-151	2/10	11 ± 23^5-151
Anti-CD154 + depleting anti-CD8	10/10	61 ± 7^5-150	10/10	72 ± 9^5-150

B6 mice were irradiated with 200 cGy TBI on day − 1 and infused with 40 × 10⁶ BALB/c BM on day 0. Anti-CD154 mAb was administered daily from day − 1 through day 5, then twice a week until day 14 at 200 μg/dose. Four hundred micrograms depleting anti-CD4, anti-CD8, or both was given on days − 2, 1, 5, and 9. PBLs were typed for percentage donor-host chimerism on days 44 and 119. n = 10 per group. Chimeric indicates number of mice with more than 3% donor cells. Shown is average percentage donor ± 1 SD of all mice in group.

ND indicates not done; for other abbreviations, see Table 1.

F5-150

P ≤ .002 compared to untreated controls.

F5-151

P ≤ .003 compared to mice receiving only anti-CD154 mAb.

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