NH2 terminal amino acid sequence analysis of the thrombin–antithrombin III complex
| Cycle . | Prothrombin . | Antithrombin III . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Position . | PTH amino acid . | pmol . | Position . | PTH amino acid . | pmol . | Position . | PTH amino acid . | pmol . | |
| 1 | 1 | Ala | 6.44 | 1 | His | 2.78 | 394 | Ser | 4.25 |
| 2 | 2 | Asn | 6.26 | 2 | Gly | 5.64 | 395 | Leu | 7.36 |
| 3 | 3 | Thr | 5.60 | 3 | Ser | 4.08 | 396 | Asn | 8.20 |
| 4 | 4 | Phe | 7.51 | 4 | Pro | 9.55 | 397 | Pro | 9.55 |
| 5 | 5 | Leu | 8.38 | 5 | Val | 5.85 | 398 | Asn | 7.12 |
| 6 | 6 | Glu | —* | 6 | Asp | 5.08 | 399 | Arg | 7.74 |
| 7 | 7 | Glu | —* | 7 | Ile | 7.07 | 400 | Val | 8.76 |
| 8 | 8 | Val | 8.76 | 8 | Cys | —* | 401 | Thr | 5.85 |
| 9 | 9 | Arg | 8.24 | 9 | Thr | 5.21 | 402 | Phe | 7.84 |
| 10 | 10 | Lys | 16.84 | 10 | Ala | 7.29 | 403 | Lys | 16.84 |
| Cycle . | Prothrombin . | Antithrombin III . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Position . | PTH amino acid . | pmol . | Position . | PTH amino acid . | pmol . | Position . | PTH amino acid . | pmol . | |
| 1 | 1 | Ala | 6.44 | 1 | His | 2.78 | 394 | Ser | 4.25 |
| 2 | 2 | Asn | 6.26 | 2 | Gly | 5.64 | 395 | Leu | 7.36 |
| 3 | 3 | Thr | 5.60 | 3 | Ser | 4.08 | 396 | Asn | 8.20 |
| 4 | 4 | Phe | 7.51 | 4 | Pro | 9.55 | 397 | Pro | 9.55 |
| 5 | 5 | Leu | 8.38 | 5 | Val | 5.85 | 398 | Asn | 7.12 |
| 6 | 6 | Glu | —* | 6 | Asp | 5.08 | 399 | Arg | 7.74 |
| 7 | 7 | Glu | —* | 7 | Ile | 7.07 | 400 | Val | 8.76 |
| 8 | 8 | Val | 8.76 | 8 | Cys | —* | 401 | Thr | 5.85 |
| 9 | 9 | Arg | 8.24 | 9 | Thr | 5.21 | 402 | Phe | 7.84 |
| 10 | 10 | Lys | 16.84 | 10 | Ala | 7.29 | 403 | Lys | 16.84 |
PTH indicates phenylthiohydantoin.
Picomole amounts of residual amino acids were at or below background levels.