CAFCs on day 20 of culture from sorted CD34+cells derived from rhesus monkey ES cells
| Cells (% of CD34+ cells) . | No. of cells plated . | CAFC count . | CAFCs/50 000 cells . |
|---|---|---|---|
| Unsorted (52%) | 50 000 | 11 | 11 |
| 50 000 | 12 | 12 | |
| Sorted (98.9%) | 5000 | 2 | 20 |
| 10 000 | 4 | 20 | |
| 20 000 | 5 | 13 | |
| 25 000 | 12 | 24 |
| Cells (% of CD34+ cells) . | No. of cells plated . | CAFC count . | CAFCs/50 000 cells . |
|---|---|---|---|
| Unsorted (52%) | 50 000 | 11 | 11 |
| 50 000 | 12 | 12 | |
| Sorted (98.9%) | 5000 | 2 | 20 |
| 10 000 | 4 | 20 | |
| 20 000 | 5 | 13 | |
| 25 000 | 12 | 24 |
Rhesus monkey ES cells underwent differentiation in the presence of S17 stromal cells, BMP-4, and hematopoietic growth factors (SCF, IL-3, IL-6, G-CSF, VEGF, Flt3 ligand, and Epo). On day 14, hematopoietic-like blast cells were harvested and sorted for CD34+ cells. The CD34+ cells were replated on S17 stromal cell layers in the presence of 20 ng/mL SCF, IL-3, G-CSF, GM-CSF, and VEGF, 10 ng/mL Flt3 ligand and IL-6, and 2 U/mL Epo.