Detection of donor HLA class II genotypes after transfusion to recipient NLR-1
| HLA allele . | Recipient NLR-1 . | Donor 1A M, 10 days . | Donor 1B F, 7 days . | Donor 1C M, 12 days . | Donor 1D F, 12 days . | Recipient, posttransfusion analysis day . |
|---|---|---|---|---|---|---|
| Transfusion . | T1 . | T1 . | T2 . | T2 . | T2 day 7 (=T1 day 20) . | |
| DR1 | POS | POS | POS | NT | ||
| DR11 | POS | NT | ||||
| DQB501 | POS | POS | POS | POS | NT | |
| DQB7 | POS | NT | ||||
| DR3 | POS | Detected | ||||
| DQB201 | POS | POS | POS | Detected | ||
| DR7 | POS | POS | POS | NT | ||
| DR4 | POS | NT | ||||
| DQB603 | POS | NT | ||||
| DQB8 | POS | NT |
| HLA allele . | Recipient NLR-1 . | Donor 1A M, 10 days . | Donor 1B F, 7 days . | Donor 1C M, 12 days . | Donor 1D F, 12 days . | Recipient, posttransfusion analysis day . |
|---|---|---|---|---|---|---|
| Transfusion . | T1 . | T1 . | T2 . | T2 . | T2 day 7 (=T1 day 20) . | |
| DR1 | POS | POS | POS | NT | ||
| DR11 | POS | NT | ||||
| DQB501 | POS | POS | POS | POS | NT | |
| DQB7 | POS | NT | ||||
| DR3 | POS | Detected | ||||
| DQB201 | POS | POS | POS | Detected | ||
| DR7 | POS | POS | POS | NT | ||
| DR4 | POS | NT | ||||
| DQB603 | POS | NT | ||||
| DQB8 | POS | NT |
Allele-specific primers and probes were designed to detect HLA genotypes after transfusion that were not present in the recipient before transfusion. Donor gender and age of RBC component at time of transfusion are listed (eg, M, 10 days). Each recipient's genotype is indicated in the second column; corresponding genes found in the transfused blood donors are aligned with the recipient's.
POS, positive for gene; detected (not detected), identified (not identified) by respective primer/probe analysis of recipient's posttransfusion sample; NT, not tested.