Frequency and type of clonogenic cells arising in response to Mpl signaling
| Experiment . | Day . | Frequency of clonogenic cells . | G . | GM . | Macro . | Mix . | Meg . | Other . |
|---|---|---|---|---|---|---|---|---|
| 1 | 7 | 59 | 58 | 15 | 4 | 11.5 | 0 | 11.5 |
| 23 | 6 250 | 0 | 0 | 0 | 0 | 100 | 0 | |
| 35 | 7 083 | 0 | 0 | 0 | 0 | 100 | 0 | |
| 77 | 42 500 | 0 | 0 | 0 | 0 | 100 | 0 | |
| 102 | 42 500 | 0 | 0 | 0 | 0 | 100 | 0 | |
| 2 | 7 | 89 | 53 | 20 | 0 | 13 | 7 | 7 |
| 32 | 16 958 | 0 | 0 | 0 | 0 | 100 | 0 | |
| 87 | 50 875 | 0 | 0 | 0 | 0 | 100 | 0 |
| Experiment . | Day . | Frequency of clonogenic cells . | G . | GM . | Macro . | Mix . | Meg . | Other . |
|---|---|---|---|---|---|---|---|---|
| 1 | 7 | 59 | 58 | 15 | 4 | 11.5 | 0 | 11.5 |
| 23 | 6 250 | 0 | 0 | 0 | 0 | 100 | 0 | |
| 35 | 7 083 | 0 | 0 | 0 | 0 | 100 | 0 | |
| 77 | 42 500 | 0 | 0 | 0 | 0 | 100 | 0 | |
| 102 | 42 500 | 0 | 0 | 0 | 0 | 100 | 0 | |
| 2 | 7 | 89 | 53 | 20 | 0 | 13 | 7 | 7 |
| 32 | 16 958 | 0 | 0 | 0 | 0 | 100 | 0 | |
| 87 | 50 875 | 0 | 0 | 0 | 0 | 100 | 0 |
Marrow cells were transduced using the F36VMpl vector, then cultured in suspension at densities of 10, 25, 50, and 100 cells per well in 96-well plates without added cytokines and in the presence of AP20187 (100 nM). The frequency and type of clones emerging in response to AP20187-triggered Mpl signaling were determined on the days indicated. Percentages of clones containing granulocytes (G), granulocyte–macrophages (GM), macrophages (Macro), mixed cells (mix), megakaryocytes (Meg), and unclassified (other) are shown. Clonogenic cell frequency indicates the number of cells plated per clonogenic cell.