Summary of experiments performed using the F1Mpl and F36VMpl vectors
| Experiment . | Duration of culture (wk) . | CFU-C . | CFU-S . |
|---|---|---|---|
| F1mpl | |||
| 1 | 38 (s) | +(220) | +(NQ) |
| 2 | 14 (d) | +(206) | +(NQ) |
| 3 | 19 (d) | +(76) | — |
| 4 | 37 (s) | +(NQ) | +(NQ) |
| 5 | 21 (d) | +(5) | +(NQ) |
| 6 | 16 (s) | +(17) | ND |
| 7 | 40 (d) | +(35) | ND |
| F36Vmpl | |||
| 1 | 9 (s) | — | — |
| 2 | 14 (s) | +(1) | ND |
| 3 | 16 (s) | ND | ND |
| 4 | 49 (s) | +(157) | ND |
| 5 | 32 (s) | +(87) | +(15.3) |
| Experiment . | Duration of culture (wk) . | CFU-C . | CFU-S . |
|---|---|---|---|
| F1mpl | |||
| 1 | 38 (s) | +(220) | +(NQ) |
| 2 | 14 (d) | +(206) | +(NQ) |
| 3 | 19 (d) | +(76) | — |
| 4 | 37 (s) | +(NQ) | +(NQ) |
| 5 | 21 (d) | +(5) | +(NQ) |
| 6 | 16 (s) | +(17) | ND |
| 7 | 40 (d) | +(35) | ND |
| F36Vmpl | |||
| 1 | 9 (s) | — | — |
| 2 | 14 (s) | +(1) | ND |
| 3 | 16 (s) | ND | ND |
| 4 | 49 (s) | +(157) | ND |
| 5 | 32 (s) | +(87) | +(15.3) |
(d) indicates cells died at the indicated time-point of culture; (s), cultures were stopped at the time-point indicated. (d) cultures died at the time point indicated. Plus signs indicate that cells were capable of generating colonies in semisolid media in the presence of IL-3 (CFU-C) and in the spleens of irradiated mice (CFU-S). Numbers in parentheses indicate clonogenic cells per 104 (for CFU-C) or 107 (for CFU-S) cells plated. Minus signs indicate cells were unable to generate either CFU-C or CFU-S. NQ, not quantified; ND, not done.