Table 1.

Comparison of the induction of NSE-positive HL60 cells by 1,25D3 or by cotransfection of cyclin-dependent kinase 5 and p35Nck5a

Treatment*NSE-positive cells (%)Significance (P value)
None 4.4  ±  0.8 — 
atRA 2.5  ±  0.8 .26 
1,25D3 27.5  ±  4.2 .02  
pEGFPN1 − Cdk5 7.0  ±  0.61-153 —  
pEGFPN1 + p35 10.0  ±  0.31-153 .43  
pEGFPN1 − Cdk5 + p35 31.2  ±  0.81-153 < .01 
Treatment*NSE-positive cells (%)Significance (P value)
None 4.4  ±  0.8 — 
atRA 2.5  ±  0.8 .26 
1,25D3 27.5  ±  4.2 .02  
pEGFPN1 − Cdk5 7.0  ±  0.61-153 —  
pEGFPN1 + p35 10.0  ±  0.31-153 .43  
pEGFPN1 − Cdk5 + p35 31.2  ±  0.81-153 < .01 

NSE indicates nonspecific esterase; atRA, all-trans retinoic acid; Cdk5, cyclin-dependent kinase 5.

*

Cells examined at 24 hours after initiation of treatment or transfection.

Mean ± SD; n = 4.

Significance was determined by 2-tailed Studentt test, comparing untreated versus treated cells in the top 3 rows, and Cdk5 transfected cells versus p35, or p35 and Cdk5 cotransfected cells, in the bottom 3 rows.

F1-153

Percentage of transfected cells as determined by green fluorescence.

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