Comparison of the induction of NSE-positive HL60 cells by 1,25D3 or by cotransfection of cyclin-dependent kinase 5 and p35Nck5a
Treatment* . | NSE-positive cells (%)† . | Significance‡ (P value) . |
---|---|---|
None | 4.4 ± 0.8 | — |
atRA | 2.5 ± 0.8 | .26 |
1,25D3 | 27.5 ± 4.2 | .02 |
pEGFPN1 − Cdk5 | 7.0 ± 0.61-153 | — |
pEGFPN1 + p35 | 10.0 ± 0.31-153 | .43 |
pEGFPN1 − Cdk5 + p35 | 31.2 ± 0.81-153 | < .01 |
Treatment* . | NSE-positive cells (%)† . | Significance‡ (P value) . |
---|---|---|
None | 4.4 ± 0.8 | — |
atRA | 2.5 ± 0.8 | .26 |
1,25D3 | 27.5 ± 4.2 | .02 |
pEGFPN1 − Cdk5 | 7.0 ± 0.61-153 | — |
pEGFPN1 + p35 | 10.0 ± 0.31-153 | .43 |
pEGFPN1 − Cdk5 + p35 | 31.2 ± 0.81-153 | < .01 |
NSE indicates nonspecific esterase; atRA, all-trans retinoic acid; Cdk5, cyclin-dependent kinase 5.
Cells examined at 24 hours after initiation of treatment or transfection.
Mean ± SD; n = 4.
Significance was determined by 2-tailed Studentt test, comparing untreated versus treated cells in the top 3 rows, and Cdk5 transfected cells versus p35, or p35 and Cdk5 cotransfected cells, in the bottom 3 rows.
Percentage of transfected cells as determined by green fluorescence.