Table 1. Comparison of the induction of... | American Society of Hematology

Table 1.

Comparison of the induction of NSE-positive HL60 cells by 1,25D₃ or by cotransfection of cyclin-dependent kinase 5 and p35Nck5a

Treatment^*	NSE-positive cells (%)^†	Significance^‡ (P value)
None	4.4 ± 0.8	—
atRA	2.5 ± 0.8	.26
1,25D₃	27.5 ± 4.2	.02
pEGFPN1 − Cdk5	7.0 ± 0.6^1-153	—
pEGFPN1 + p35	10.0 ± 0.3^1-153	.43
pEGFPN1 − Cdk5 + p35	31.2 ± 0.8^1-153	< .01

Treatment^*	NSE-positive cells (%)^†	Significance^‡ (P value)
None	4.4 ± 0.8	—
atRA	2.5 ± 0.8	.26
1,25D₃	27.5 ± 4.2	.02
pEGFPN1 − Cdk5	7.0 ± 0.6^1-153	—
pEGFPN1 + p35	10.0 ± 0.3^1-153	.43
pEGFPN1 − Cdk5 + p35	31.2 ± 0.8^1-153	< .01

NSE indicates nonspecific esterase; atRA, all-trans retinoic acid; Cdk5, cyclin-dependent kinase 5.

Cells examined at 24 hours after initiation of treatment or transfection.

†

Mean ± SD; n = 4.

‡

Significance was determined by 2-tailed Studentt test, comparing untreated versus treated cells in the top 3 rows, and Cdk5 transfected cells versus p35, or p35 and Cdk5 cotransfected cells, in the bottom 3 rows.

F1-153

Percentage of transfected cells as determined by green fluorescence.

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