Reconstitution of human lymphohematopoiesis in nonobese diabetic/severe combined immunodeficiency mice given transplants of CD34+CD81+, CD34lowCD81+, and CD34+CD81high cells
| Phenotype of cells transplanted . | Mice with reconstitution (no.)/mice given transplants (no.) . | Human CD45+ cells in BM of mice with reconstitution (%) . | Lineage markers expressed in CD45+ cells (%) . | ||||
|---|---|---|---|---|---|---|---|
| CD19/CD10 . | CD13 . | CD33 . | CD34 . | CD81 . | |||
| CD34+CD81+ | 9/13 | 29.3 ± 23.1 | 80.0 ± 18.0 | 19.5 ± 18.2 | 21.4 ± 15.4 | 5.4 ± 2.7 | 92.7 ± 6.0 |
| CD34lowCD81+ | 0/9 | — | — | — | — | — | — |
| CD34+CD81high | 0/9 | — | — | — | — | — | — |
| Phenotype of cells transplanted . | Mice with reconstitution (no.)/mice given transplants (no.) . | Human CD45+ cells in BM of mice with reconstitution (%) . | Lineage markers expressed in CD45+ cells (%) . | ||||
|---|---|---|---|---|---|---|---|
| CD19/CD10 . | CD13 . | CD33 . | CD34 . | CD81 . | |||
| CD34+CD81+ | 9/13 | 29.3 ± 23.1 | 80.0 ± 18.0 | 19.5 ± 18.2 | 21.4 ± 15.4 | 5.4 ± 2.7 | 92.7 ± 6.0 |
| CD34lowCD81+ | 0/9 | — | — | — | — | — | — |
| CD34+CD81high | 0/9 | — | — | — | — | — | — |
Freshly sorted CD34+CD81+, CD34lowCD81+, or CD34+CD81high cells were inserted into nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice (1 × 104 cells in each recipient). Mice were killed 12 to 16 weeks afterward and their bone marrow (BM) cells were analyzed by flow cytometry.