Table 2. Comparison of components of... | American Society of Hematology

Table 2.

Comparison of components of mixed hematopoietic colonies derived from CD34⁺CD81⁺ and CD34^lowCD81⁺ cells

Colony	CD34⁺CD81⁺			CD34^lowCD81⁺
Colony	Erythroid (%)	Myeloid (%)	Megakaryocytes	Erythroid (%)	Myeloid (%)	Megakaryocytes
1	72.9	27.1	−	98.2	1.8	+
2	64.4	35.6	−	99.6	0.4	+
3	75.7	24.3	−	98.3	1.7	+
4	62.2	37.8	−	99.5	0.5	+
5	87.9	12.1	+	96.8	3.2	+
6	65.7	34.3	−	98.0	2.0	+
7	86.5	13.5	−	96.3	3.7	+
8	88.2	11.8	−	97.1	2.9	+
9	90.5	9.5	+	96.7	3.3	+
10	61.1	38.9	−	94.2	5.8	+
Mean ± SD	75.5 ± 11.9	24.5 ± 11.9	2/10	97.5 ± 1.6^*	2.5 ± 1.6^*	10/10

Colony	CD34⁺CD81⁺			CD34^lowCD81⁺
Colony	Erythroid (%)	Myeloid (%)	Megakaryocytes	Erythroid (%)	Myeloid (%)	Megakaryocytes
1	72.9	27.1	−	98.2	1.8	+
2	64.4	35.6	−	99.6	0.4	+
3	75.7	24.3	−	98.3	1.7	+
4	62.2	37.8	−	99.5	0.5	+
5	87.9	12.1	+	96.8	3.2	+
6	65.7	34.3	−	98.0	2.0	+
7	86.5	13.5	−	96.3	3.7	+
8	88.2	11.8	−	97.1	2.9	+
9	90.5	9.5	+	96.7	3.3	+
10	61.1	38.9	−	94.2	5.8	+
Mean ± SD	75.5 ± 11.9	24.5 ± 11.9	2/10	97.5 ± 1.6^*	2.5 ± 1.6^*	10/10

Ten mixed hematopoietic colonies derived from either CD34⁺CD81⁺ or CD34^lowCD81⁺ cells were chosen randomly from semisolid cultures on day 10 of culture. Clonal cells were individually centrifuged on slides and then stained with May-Grünwald-Giemsa solution. The percentages shown were obtained by counting 300 cells in one sample in at least 5 observation views. For CD34⁺CD81⁺ cells, mean ± SD values were 75.5% ± 11.9% and 24.5% ± 11.9%, respectively, for erythroid and myeloid colonies. For CD34^lowCD81⁺ cells, values were 97.5% ± 1.6% and 2.5% ± 1.6%, respectively, for erythroid and myeloid colonies.

P < .001 compared with the CD34⁺CD81⁺ cell population for each colony type.

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