Table 1. Colony formation from human... | American Society of Hematology

Table 1.

Colony formation from human cord blood mononuclear cells defined by expression of CD34 and CD81

Phenotype of cultured cells	Sorted cells in total CD34⁺ cells (%)	Granulocytes	Macrophages	Granulocytes-macrophages	Erythroid bursts	Mixed hematopoietic	Megakaryocytes	Total
CD34⁺CD81⁺	44.6 ± 8.1	2.8 ± 1.1	7.7 ± 1.8	27.1 ± 8.0	2.5 ± 1.3	15.5 ± 7.7	0	65.4 ± 10.1
CD34^lowCD81⁺	19.2 ± 6.0	0.6 ± 0.7^*	0.1 ± 0.3^*	0.3 ± 0.3^*	3.0 ± 2.8	27.9 ± 9.1^†	3.6 ± 0.6^*	35.7 ± 10.8^*
CD34⁺CD81^high	6.4 ± 5.1	0	0	0	0	0	0	0
CD34⁺^‡	—	2.6 ± 1.9	10.7 ± 2.0	19.5 ± 6.9	2.3 ± 1.6	7.8 ± 2.8	0.6 ± 0.2	41.1 ± 5.2

Phenotype of cultured cells	Sorted cells in total CD34⁺ cells (%)	Granulocytes	Macrophages	Granulocytes-macrophages	Erythroid bursts	Mixed hematopoietic	Megakaryocytes	Total
CD34⁺CD81⁺	44.6 ± 8.1	2.8 ± 1.1	7.7 ± 1.8	27.1 ± 8.0	2.5 ± 1.3	15.5 ± 7.7	0	65.4 ± 10.1
CD34^lowCD81⁺	19.2 ± 6.0	0.6 ± 0.7^*	0.1 ± 0.3^*	0.3 ± 0.3^*	3.0 ± 2.8	27.9 ± 9.1^†	3.6 ± 0.6^*	35.7 ± 10.8^*
CD34⁺CD81^high	6.4 ± 5.1	0	0	0	0	0	0	0
CD34⁺^‡	—	2.6 ± 1.9	10.7 ± 2.0	19.5 ± 6.9	2.3 ± 1.6	7.8 ± 2.8	0.6 ± 0.2	41.1 ± 5.2

Cells sorted from freshly harvested human cord blood mononuclear cells (CB MNC) (100 cells from each fragment) were cultured in the presence of stem cell factor, interleukin 3 (IL-3), IL-6, granulocyte colony-stimulating factor, thrombopoietin, and erythropoietin. Colonies were counted on days 7 to 14 of cultures. Data are mean (±SD) values from 6 independent experiments.

P < .01 compared with the CD34⁺CD81⁺ population.

†

P < .05 compared with the CD34⁺CD81⁺ population.

‡

CD34⁺ cells were separated by using the Dynal (Oslo, Norway) CD34 progenitor cell–selection system and cultured under the same conditions, along with sorted cells as the control (n = 3). The purity of harvested CD34⁺ cells was more than 92% in all 3 experiments.

View Large

This Feature Is Available To Subscribers Only