Diversity of subclones found in follicular lymphoma 8-'83
| Isotype . | No. of clone sequences . | Total no. of intraclonal nucleotide differences* . | Intraclonal variation (no. of mutations/clone) . |
|---|---|---|---|
| 8-'83 IgM | 22 | 59 | 2.7 |
| 8-'83 IgG | 20 | 61 | 3.1 |
| 8-'92 IgG | 61-153 | 8 | 1.3 |
| Isotype . | No. of clone sequences . | Total no. of intraclonal nucleotide differences* . | Intraclonal variation (no. of mutations/clone) . |
|---|---|---|---|
| 8-'83 IgM | 22 | 59 | 2.7 |
| 8-'83 IgG | 20 | 61 | 3.1 |
| 8-'92 IgG | 61-153 | 8 | 1.3 |
IgM, immunoglobulin M; IgG, immunoglobulin G.
The majority of the nucleotide differences found were mutations that were shared by more than one clone, which argues strongly for somatic hypermutation rather than Taq errors.
Clones were not obtained by microdissection of samples, but bacterial clones were made from the VH3–polymerase chain reaction product derived from 8-'92 complementary DNA.10