Cytotoxic T-lymphocyte activation by different types of dendritic cells
| Method of transfection . | CTL activation (IU IFN-γ/mL/24 h) . | |||
|---|---|---|---|---|
| iMo-DCs . | mMo-DCs . | 34-LCs . | 34-DCs . | |
| Electroporation | 11.3 ± 2.2 | 5.8 ± 1.8 | 6.9 ± 1.4 | 7.7 ± 3 |
| Lipofection | 3.7 ± 1.1 | 1.5 ± 0.8 | <BG | <BG |
| Passive pulsing | <BG | <BG | <BG | <BG |
| Method of transfection . | CTL activation (IU IFN-γ/mL/24 h) . | |||
|---|---|---|---|---|
| iMo-DCs . | mMo-DCs . | 34-LCs . | 34-DCs . | |
| Electroporation | 11.3 ± 2.2 | 5.8 ± 1.8 | 6.9 ± 1.4 | 7.7 ± 3 |
| Lipofection | 3.7 ± 1.1 | 1.5 ± 0.8 | <BG | <BG |
| Passive pulsing | <BG | <BG | <BG | <BG |
Different types of dendritic cells (DCs) were transfected with in vitro-transcribed Melan mRNA using electroporation, lipofection, or passive pulsing. One day after transfection, 105 transfected DCs were cocultured for 24 hours with 105 Melan-A-specific cytotoxic T lymphocytes (CTLs) at 37°C. Afterwards, supernatants were collected and IFN-γ secretion was checked by IFN-γ enzyme-linked immunosorbant assay (ELISA), as described in “Materials and methods.” Results are the mean ± SD of at least 5 independent experiments for electroporation and of 3 independent experiments for passive pulsing and lipofection. iMo-DCs, immature Mo-DCs; mMo-DCs, mature Mo-DCs; 34-LCs, CD34+ progenitor-derived Langerhans cells; 34-DCs, CD34+ progenitor-derived DCs; <BG, IFN-γ production below background.