Efficiency of mRNA transfection in different types of dendritic cells
| Method of transfection . | Transfection efficiency (%) . | |||
|---|---|---|---|---|
| iMo-DCs . | mMo-DCs . | 34-LCs . | 34-DCs . | |
| Electroporation | 63 ± 9 | 33 ± 8 | 50 ± 3 | 73 ± 3 |
| Lipofection | 7.5 ± 2.5 | 4 ± 2.3 | <BG | <BG |
| Passive pulsing | <BG | <BG | <BG | <BG |
| Method of transfection . | Transfection efficiency (%) . | |||
|---|---|---|---|---|
| iMo-DCs . | mMo-DCs . | 34-LCs . | 34-DCs . | |
| Electroporation | 63 ± 9 | 33 ± 8 | 50 ± 3 | 73 ± 3 |
| Lipofection | 7.5 ± 2.5 | 4 ± 2.3 | <BG | <BG |
| Passive pulsing | <BG | <BG | <BG | <BG |
Different types of dendritic cells (DCs) were transfected with in vitro–transcribed (IVT) enhanced green fluorescent protein (EGFP) mRNA using electroporation, lipofection, or passive pulsing. One day after transfection, EGFP expression was analyzed by flow cytometric analysis (FCM) to estimate transfection efficiency (% EGFP+ DCs). iMo-DCs, immature Mo-DCs; mMo-DCs, mature Mo-DCs; 34-LCs, CD34+ progenitor-derived Langerhans cells; 34-DCs, CD34+ progenitor-derived dendritic cells; <BG, EGFP expression below background fluorescence. Results are the mean ± standard deviation (SD) of at least 3 independent experiments for passive pulsing, lipofection, or electroporation.