Optimization of mRNA electroporation parameters in K562 cells
| . | Electroporation . | ||||
|---|---|---|---|---|---|
| Voltage (V) . | Capacitance (μF) . | Cell volume (μL) . | Efficiency (%) . | Viability (%) . | |
| DNA | 260 | 1050 | 500 | 40 | 49 |
| 300 | 150 | 200 | 28 | 85 | |
| RNA | 300 | 150 | 200 | 89 | 85 |
| 450 | 150 | 200 | 85 | 37 | |
| 300 | 300 | 200 | 83 | 59 | |
| 260 | 1050 | 500 | 81 | 73 | |
| 250 | 1500 | 500 | 80 | 69 | |
| . | Electroporation . | ||||
|---|---|---|---|---|---|
| Voltage (V) . | Capacitance (μF) . | Cell volume (μL) . | Efficiency (%) . | Viability (%) . | |
| DNA | 260 | 1050 | 500 | 40 | 49 |
| 300 | 150 | 200 | 28 | 85 | |
| RNA | 300 | 150 | 200 | 89 | 85 |
| 450 | 150 | 200 | 85 | 37 | |
| 300 | 300 | 200 | 83 | 59 | |
| 260 | 1050 | 500 | 81 | 73 | |
| 250 | 1500 | 500 | 80 | 69 | |
K562 cells were electroporated as described in “Materials and methods.” Cells were analyzed 24 hours after electroporation by flow cytometric analysis for enhanced green fluorescent protein (EGFP) expression to estimate transfection efficiency (= % EGFP+cells) as well as by ethidium bromide exclusion for cell viability. Results are the mean of 4 independent experiments each with a different in vitro–transcribed mRNA batch (standard error of the mean < 2.5%).