Time course of the generation of LTR-HSCs from 8.5 dpc P-Sp and YS (2 embryo equivalents) in cocultures with AGM-S3 cells
| Cell source . | No. of mice reconstituted/transplanted . | ||
|---|---|---|---|
| Day 2 . | Day 3 . | Day 4 . | |
| P-Sp | 0/4 | 0/4 | 9/11 |
| YS | 0/4 | 0/4 | 5/10 |
| Cell source . | No. of mice reconstituted/transplanted . | ||
|---|---|---|---|
| Day 2 . | Day 3 . | Day 4 . | |
| P-Sp | 0/4 | 0/4 | 9/11 |
| YS | 0/4 | 0/4 | 5/10 |
Cells cultured from 8.5 dpc P-Sp and YS cells of Ly-5.2+ mice were transplanted into Ly-5.1+mice, and 12 weeks later Ly-5.2+ B lymphocytes (B220+), T lymphocytes (Thy-1+), and myeloid cells (Gr-1+/Mac-1+) were analyzed by flow cytometry. The mice in which Ly-5.2+ cells made up more than 1% of all B220+, Thy-1+, and Gr-1+/Mac-1+ cells in PB were scored as reconstituted mice.